Rat LAMP1 / CD107a Protein

Lysosomal-associated membrane protein 1 Protein Datasheet

LAMP1 / CD107a Protein Product Information

• Synonym: LAMP1
• Protein Construction: A DNA sequence encoding the Rat LAMP1 (P14562) (Met1-Asn371) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

LAMP1 / CD107a Protein QC Testing

• Purity: >92 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 22
• Molecular Mass: The recombinant Rat LAMP1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 591 amino acids and has a predicted molecular mass of 65.1 kDa. The apparent molecular mass of the protein is approximately 123 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: LAMP1 / CD107a protein

LAMP1 / CD107a Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

LAMP1 / CD107a Protein Description

Lysosome-associated membrane glycoprotein 1, also known as CD107 antigen-like family member A, CD107a, and LAMP1, is a single-pass type I  membrane protein which belongs to the LAMP family. CD107a is expressed largely in the endosome-lysosome membranes of cells, but is also found on the plasma membrane (1-2% of total LAMP1). LAMP1 has been implicated in a variety of cellular functions, including cancer metastasis. It has been proposed LAMP1 serves as a therapeutic agent for some cancers, as well as a marker for lysosomal storage disorders and different cell types such as cytotoxic T cells. LAMP2, also known as CD107b, may also play a role in tumor cell metastasis and functions in the protection, maintenance, and adhesion of the lysosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

References

1. Schleutker J. et al., 1992, Hum Genet. 88 (1): 95-7.
2. Kannan K.et al., 1996, Cell Immunol. 171 (1):10-9.
3. Chang MH. et al., 2003, J Biol Regul Homeost Agents. 16 (2): 147-51.
4. Parkinson-Lawrence EJ. et al., 2005, Cell Immunol. 236 (1-2): 161-6.