LAIR2 Antibody ( FITC )

LAIR2 Antibody Datasheet

LAIR2 Antibody Product Information

• Immunogen: Recombinant Human LAIR2 protein (Catalog#10868-H08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 024
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human LAIR2 (rh LAIR2; Catalog#10868-H08H; NP_002279.2; Met 1-Pro 152) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

LAIR2 Antibody Usage Guide

• Specificity: Human LAIR2
• Flow Cytometry:
  

  Flow cytometric analysis of Human LAIR2(CD306) expression in Jurkat cells. The cells were treated according to manufacturer's manual (BD Pharmingen™ Cat. No. 554714), and then stained with FITC Rabbit anti-LAIR2(CD306) (10868-R024-F). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

LAIR2 Antibody Background

Leukocyte-associated immunoglobulin-like receptor 2 (LAIR2), also known as CD306, is a 131 amino acid protein containing one lg-like C2-type domain. It is expressed as a soluble receptor exhibiting high affinity for various collagen molecules to which it binds in a hydroxyproline-dependent manner. LAIR2 is a member of the immunoglobulin superfamily and was identified by its similarity to LAIR1, an inhibitory receptor present on mononuclear leukocytes. LAIR2 is thought to be secreted and may help modulate mucosal tolerance. As a natural competitor for LAIR1, soluble LAIR2 prevents binding of human LAIR1 to collagens and LAIR1 cross-linking, thereby regulating its inhibitory potential. LAIR2 is suggested to perform an immunoregulatory function.

References

1. Meyaard, L. et al.,1997, Immunity.7: 283-290.
2. Meyaard, L. et al., 2001, J. Exp. Med. 194 (1): 107-112.
3. Meyaard, L., 2003, J Biol Regul Homeost Agents. 17 (4): 330-333.
4. Lebbink, RJ. et al., 2008, J Immunol. 180 (3): 1662-1669.
5. Lebbink, RJ. et al., 2009, Matrix Biol. 28 (4): 202-210.