|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus leukocyte-associated immunoglobulin-like receptor 1 DNA.|
|Identical with the Gene Bank Ref. ID sequence except for the point mutations: 499 C/T，605 T/C, resulting in the amino acid Arg substitution by Cys, Met substitution by Thr and 711 A/G not causing the amino acid variation.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat LAIR1 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80415-G-F|
|Rat LAIR1 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80415-G-H|
|Rat LAIR1 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80415-G-M|
|Rat LAIR1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80415-G-N|
|Rat LAIR1 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80415-G-Y|
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Leukocyte associated Ig-like receptor-1 (LAIR1) is a surface molecule expressed on human mononuclear leukocytes that functions as an inhibitory receptor on human NK cells. In addition to NK cells, LAIR1 is expressed on T cells, B cells, macrophages, and dendritic cells. It is predicted to mediate inhibitory functions based on the presence of immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in its cytoplasmic domain. Cross-linking of LAIR1 on human T cell clones results in inhibition of cytotoxicity only in T cell clones that lack CD28 and are able to spontaneously lyse certain targets in vitro. Moreover, the cytolytic activity of freshly isolated T cells, which is thought to be mainly due to "effector" T cells, can be inhibited by anti-LAIR1 mAb. Thus, LAIR1 functions as an inhibitory receptor not only on NK cells, but also on human T cells. This indicates that LAIR1 provides a mechanism of regulation of effector T cells and may play a role in the inhibition of unwanted bystander responses mediated by Ag-specific T cells.