Rat LAIR1 Gene cDNA Clone / ORF Clone

LAIR1 cDNA Clone Datasheet

LAIR1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001029928.1
• cDNA Size: 792
• Gene Synonym: Lair1
• Source: Rat

LAIR1 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus leukocyte-associated immunoglobulin-like receptor 1 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 499 C/T，605 T/C, resulting in the amino acid Arg substitution by Cys, Met substitution by Thr and 711 A/G not causing the amino acid variation.

LAIR1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LAIR1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LAIR1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGCCACTTC ATTCTGTCAT CGTGCTGGTG CTTGTACTGT GCCTGGGATG GAAAAGTAAC
61       ACACAGGAGG AATCTCTGTC CGATTTCACT ATCTGCGCTG AGCCAGGTCC TGTGATTCCT
121      CAAGGGAATT TCATAACCAT TGTGTGCTCA ACCTCTGGTG AATATGACAC GGTCCGCCTG
181      GAGAAGGAAG GCAGCACCTT TATGGAAAAG AAAACAGAGC CTCATGGAAA ACAGCATAGA
241      TTCCGTATTG GGCCAGTGAA TGAAACCATT ACTGGATATT ATAACTGTAT CTTTGAAAAA
301      AATTATGTAT GGTCTCAACG AAGTAATGAT CTGCAGCTAA AAGTGATCAA AGAAAATGTC
361      ACCCAGGGTC TTGCCCCAGG TCCATCAATG ACCTCAGATA CATCTTGGCT GAAGACGTAC
421      AGCATTCACA TTCTTACTGT GGTCTCTGTG ATTTTCCTCC TTTGTCTTTC CCTCTTTCTA
481      TTCTGCTTTC TTAGCCACCG TCAGAAAAAG CAGGGACTTC CAAACAATAA GAGCCAGCAT
541      CAGAGGTCAC AGGAGAGGCT CAACCTAGCT ACTAATGGCC TGGAGAAGAC ATCAGACATA
601      GTCACGGATG ACAGCCTTTC TGAGGACAGA CAGACAGAAA CTTGGACCCC AGTTGCAGGA
661      GATCTTCAGG AGGTGACATA TGCCCAGCTG GACCATGACT CCCTCACACA GAGGACTGTT
721      AAAGATGTGA CCCCACAGAA CAGAGTTATC ATGGCTGAGT CCAGTACATA TGCAGCCATC
781      ATGAGATGCT GA

 

Quality Control:cDNA sequencing results is available upon request.