Rat LAG3 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80367-G	Cloning vector ($195) pCMV / hygro ($395) pCMV / hygro - HA ($395) pCMV / hygro - His ($395) pCMV / hygro - Myc ($395) pCMV / hygro - FLAG ($395)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

LAG3 cDNA Clone Datasheet

LAG3 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_212513.2
cDNA Size:	1578
Gene Synonym:	CD223; MGC108901; Lag3
Source:	Rat

LAG3 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus lymphocyte-activation gene 3 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

LAG3 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

LAG3 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

LAG3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGCAGG ATCTGTTCCT TGACCTTTTG CTTCTGCAGC TGCTTTGGGA AGCTCCAGTT
61       GTGTCTTCAG GGCCTGGGAA AGAGCTCTCC GTGGTGTGGG CCCAGGAGGG AGCTCCTGTC
121      CATCTTCCCT GCAGCCTCGA ATTTCCCCAC CTGGATCCCA ACTTTCTGCG AAGAGGATGG
181      GTCACCTGGC AACATCGACC AGACAGTGAC CAACCCGCTT CCATCCCGGC CCTTGACCTT
241      CTCCAGGGAA TGCCCTCGAC TAGGAGACAC CCACCCCATC GCTACACGGT GCTGAGTGTG
301      GCTCCAGGAG GCCTGCGCAG CGGGAGGCAG CCCCTGCTAT CCCACGTGCA GCTGGAGAAG
361      CGTGGCCCCC AGCGCGGGGA CTTCTCTCTG TGGTTGCGCC CAGCTACGCG CAAAGATGCG
421      GGCGAGTACC ACGCCTTCGT GCGCCTCCCG GACCGCGACT TCTCCTGCAG CCTCCGCCTG
481      CGCGTCGGCC AGGCCTCGAT GATTGCCAGT CCCCCAGGAA CCCTCAAGCC GTCTGATTGG
541      GTCATTTTGA ACTGCTCCTT CAGTCGTCCT GACCGCCCAG TCTCTGTGCA CTGGTTCCAG
601      GGCCAAAGCC GAGTGCCCGT CCACAATTCA CCCCGTCATT ATCTAGCTGA AAGTTTCCTC
661      TTACTGCCCC AAGTCAGCCC ACTGGATTCC GGGACCTGGG GCTGTGTCCT CACCTACAGA
721      GATGGCTTCA ATGTCTCCAT CACGTACAAC CTCAAGGTTC AGGGTCTGGA ACCTGTAGCC
781      CCTTTGACAG TGTACGCTGC TGAAGGTTCT AGGGTGGAGC TGCCCTGTCA CTTGCCTCCC
841      GTTGTGGGGA CCCCTTCTTT GCTCATTGCC AAGTGGACTC CTCCTGGGGG AGGTCCTGAG
901      CTCCCGGTGA CTGGAAAGAG TGGCAATTTT ACCCTTCAAC TTGAGAATGT GGGTCGGGCA
961      CAGGCTGGGA CCTACACCTG CAGCATCCAT CTGCAGGGGC GGCAGCTCAG TGCGGCTGTG
1021     ACGTTGGCAG TCATCACAGT GACTCCTAAA TCCTTCGGGT TACCTGGCTC CCCGCAGAAG
1081     CTGTTATGTG AGGTAGTCCC GGCATCTGGA GAAGGAAGAT TTGTGTGGCG CCCCCTCAGC
1141     GATCTGTCCA GGAGTTCCCT GGGCCCTGTG CTGGAGTTGC AGGAGGCCAA GCTTCTGGCT
1201     GAGCAATGGC AGTGTCAGCT GTATGAGGGC CAGAAACTTC TTGGAGCAAC AGTGTACACC
1261     GCAGAGTCTA GCTCAGGCGC CTGGAGTGCT AAGAGAATCT CAGGTGACCT TAAAGGAGGC
1321     CATCTCTTCC TCTCTCTCAT CCTTGGTGCC CTTGCCTTGT TCCTCTTGGT GACCGGGGCC
1381     TTTGGCTTTC ACCTGTGGAG AAGACAGTTG CTACGGAGAA GATTTTCTGC CTTAGAGCAT
1441     GGGATTCGCC CACCTCCGGT TCAGAGTAAG ATAGAGGAGC TGGAGCGAGA ACCGGAGACC
1501     GAGATGGAAC CAGAGACAGA GCCCGATCCG GAGCCTCAGC CGGAGCCCGA GCTGGAACCA
1561     GAGTCCAGGC AGCTCTGA

Quality Control:cDNA sequencing results is available upon request.