KYNU / Kynureninase Protein (His Tag)

Kynureninase Protein Datasheet

KYNU / Kynureninase Protein Product Information

• Synonym: KYNU
• Protein Construction: A DNA sequence encoding the human KYNU (Q16719) (Met 1- Asn 465) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Baculovirus-Insect cells

KYNU / Kynureninase Protein QC Testing

• Purity: > 92 % as determined by SDS-PAGE.
• Bio-activity: Measured by its ability to oxidize 3-hydroxykynurenine.
  The specific activity is > 200 pmoles/min/μg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met
• Molecular Mass: The recombinant human KYNU consists of 475 amino acids and predicts a molecular mass of 53.7 kDa. The apparent molecular mass of rhKYNU is approximately 47 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from 0.2μm filtered solution of 20mM Tris, 500mM NaCl, pH8.0, 25% gly
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KYNU / Kynureninase protein

KYNU / Kynureninase Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KYNU / Kynureninase Protein Description

Kynureninase (KYNU, or L-kynurenine hydrolase) is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to anthranilic acid and l-alanine. Sequence alignment with other PLP-dependent enzymes indicated that kynureninase is in subgroup IVa of the aminotransferases, along with nifS, CsdB, and serine-pyruvate aminotransferase, which suggests that kynureninase has an aminotransferase fold. Kynureninase is one of the enzymes involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. belongs to the class V group of aspartate aminotransferase superfamily of structurally homologous pyridoxal 5'-phosphate (PLP) dependent enzymes.

References

1. Momany C. et al., 2004, Biochemistry. 43 (5): 1193-203.
2. Lima S. et al., 2007, Biochemistry. 46 (10): 2735-44.
3. Toma S. et al., 1997, FEBS Lett. 408 (1): 5-10.