Anti-Cadherin-16 Antibody

Cat: 10915-MM09
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Anti-Cadherin-16 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-Cadherin-16 Antibody
Validated applications
ELISA,ELISA(Det)
Species reactivity
Reacts with: Human
Specificity
Human Cadherin-16
No cross-reactivity in ELISA with Human cell lysate (293 cell line)
Immunogen
Recombinant Human KSP-Cadherin / Cadherin-16 / CDH16 protein (Catalog#10915-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human KSP-Cadherin / Cadherin-16 / CDH16 (rh KSP-Cadherin / Cadherin-16 / CDH16; Catalog#10915-H08H; NP_004053.1; Met 1-Ala 786). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #7C9A10
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Cadherin-16 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  
ELISA(Det) 1:1000-1:10000 This antibody will detect Human KSP-Cadherin / Cadherin-16 in ELISA pair set (Catalog: # SEK10915). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 10915-MM03).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Cadherin-16 Antibody Alternative Names
Anti-UNQ695/PRO1340 Antibody
Cadherin-16 Background Information

KSP-Cadherin/Cadherin-16 is a member of the cadherin superfamily, calcium-dependent, membrane-associated glycoproteins. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. KSP-Cadherin/Cadherin-16 can be detected at later stages of tubulogenesis during human renal development and in the distal tubules of adult kidneys, no expression was found by immunohistochemistry or Western blot analysis in RCC tumour tissues and several RCC cell lines. However, despite the lack of protein expression, mRNA synthesis of KSP-Cadherin/Cadherin-16 could be detected by reverse transcriptase-polymerase chain reaction analysis in all RCC tissues and most of the RCC cell lines studied, although at a reduced level. The loss of KSP-Cadherin/Cadherin-16 protein was only observed in the malignant part of the tumour kidneys, whereas in the normal part of the affected kidneys KSP-Cadherin/Cadherin-16 expression was clearly detected. These results indicate a downregulation of Ksp-cadherin in RCC and suggest a role for this cell adhesion molecule in tumour suppression.

Full Name
cadherin 16
References
  • Thomson RB, et al. (1999) Immunolocalization of Ksp-cadherin in the adult and developing rabbit kidney. Am J Physiol. 277 (1): 146-56.
  • Thedieck C, et al. (2005) Expression of Ksp-cadherin during kidney development and in renal cell carcinoma. Br J Cancer. 92(11): 2010-7.
  • Bai Y, et al. (2002) Regulation of kidney-specific Ksp-cadherin gene promoter by hepatocyte nuclear factor-1beta. Am J Physiol Renal Physiol. 283(4): 839-51.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Cadherin-16 Antibody Mouse Monoclonal ELISA(Cap) 10915-MM03
    Anti-Cadherin-16 Antibody Mouse Monoclonal IHC-P 10915-MM08
    Anti-Cadherin-16 Antibody Rabbit Polyclonal ELISA,IHC-P 10915-T24
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