KSP-Cadherin / Cadherin-16 / CDH16 Antibody

KSP-Cadherin / Cadherin-16 / CDH16 Antibody Datasheet

KSP-Cadherin / Cadherin-16 / CDH16 Antibody Product Information

• Immunogen: Recombinant Human KSP-Cadherin / Cadherin-16 / CDH16 protein (Catalog#10915-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 7C9A10
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human KSP-Cadherin / Cadherin-16 / CDH16 (rh KSP-Cadherin / Cadherin-16 / CDH16; Catalog#10915-H08H; NP_004053.1; Met 1-Ala 786). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

KSP-Cadherin / Cadherin-16 / CDH16 Antibody Usage Guide

• Specificity: Human KSP-Cadherin / Cadherin-16 / CDH16
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CDH16 in WB. Using a DAB detection system, the detection limit for Human CDH16 is approximately 20 ng/lane under reducing conditions.Use of this antibody under non-reducing conditions is not recommended.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CDH16. The detection limit for Human CDH16 is approximately 0.15 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KSP-Cadherin / Cadherin-16 / CDH16 Antibody Background

Cadherins (Calcium dependentadhesion molecules) are a class of transmembrane proteins. They play important roles incell adhesion, ensuring that cells within tissues are bound together. They are dependent on calcium (Ca2+) ions to function. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of an HAV cell adhesion recognition sequence specific to type I cadherins. It has been observed that cells containing a specific cadherin subtype tend to cluster together to the exclusion of other types, both in cell culture and during development. Cadherin-16, also known as Kidney-specific cadherin, Ksp-cadherin and CDH16, is a single-pass type I membrane protein. Cadherin-16 / CDH16 is exclusively expressed in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Cadherin-16 / CDH16 consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins.

References

1. Angst, B. et al., 2001, J Cell Sci. 114 (Pt 4): 629-41.
2. Duguay, D. et al., 2003, Dev. Biol.253: 309-23.
3. Bayas, MV. et al., 2005, Biophysical. Journal.90 (4): 1385.
4. Crosby, CV, et al., 2005,Blood. 105 (7): 2771-6.
5. Hulpiau, P. et al., 2009, Int. J. Biochem. Cell Biol.41 (2): 349-69.