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Human KRT5 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| HG13195-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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KRT5 cDNA Clone Datasheet
KRT5 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_000424.3 |
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cDNA Size: |
1773 |
| Gene Synonym: |
K5; CK5; DDD; EBS2; KRT5A;KRT5 |
| Source: | Human |
KRT5 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Homo sapiens keratin 5 DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutations: 591 C/A resulting in the amino acid Asp substitution by Glu and 594 C/A not causing the amino acid variation. |
KRT5 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
KRT5 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
KRT5 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGTCTCGCC AGTCAAGTGT GTCCTTCCGG AGCGGGGGCA GTCGTAGCTT CAGCACCGCC 61 TCTGCCATCA CCCCGTCTGT CTCCCGCACC AGCTTCACCT CCGTGTCCCG GTCCGGGGGT 121 GGCGGTGGTG GTGGCTTCGG CAGGGTCAGC CTTGCGGGTG CTTGTGGAGT GGGTGGCTAT 181 GGCAGCCGGA GCCTCTACAA CCTGGGGGGC TCCAAGAGGA TATCCATCAG CACTAGTGGT 241 GGCAGCTTCA GGAACCGGTT TGGTGCTGGT GCTGGAGGCG GCTATGGCTT TGGAGGTGGT 301 GCCGGTAGTG GATTTGGTTT CGGCGGTGGA GCTGGTGGTG GCTTTGGGCT CGGTGGCGGA 361 GCTGGCTTTG GAGGTGGCTT CGGTGGCCCT GGCTTTCCTG TCTGCCCTCC TGGAGGTATC 421 CAAGAGGTCA CTGTCAACCA GAGTCTCCTG ACTCCCCTCA ACCTGCAAAT CGACCCCAGC 481 ATCCAGAGGG TGAGGACCGA GGAGCGCGAG CAGATCAAGA CCCTCAACAA TAAGTTTGCC 541 TCCTTCATCG ACAAGGTGCG GTTCCTGGAG CAGCAGAACA AGGTTCTGGA AACAAAGTGG 601 ACCCTGCTGC AGGAGCAGGG CACCAAGACT GTGAGGCAGA ACCTGGAGCC GTTGTTCGAG 661 CAGTACATCA ACAACCTCAG GAGGCAGCTG GACAGCATCG TGGGGGAACG GGGCCGCCTG 721 GACTCAGAGC TGAGAAACAT GCAGGACCTG GTGGAAGACT TCAAGAACAA GTATGAGGAT 781 GAAATCAACA AGCGTACCAC TGCTGAGAAT GAGTTTGTGA TGCTGAAGAA GGATGTAGAT 841 GCTGCCTACA TGAACAAGGT GGAGCTGGAG GCCAAGGTTG ATGCACTGAT GGATGAGATT 901 AACTTCATGA AGATGTTCTT TGATGCGGAG CTGTCCCAGA TGCAGACGCA TGTCTCTGAC 961 ACCTCAGTGG TCCTCTCCAT GGACAACAAC CGCAACCTGG ACCTGGATAG CATCATCGCT 1021 GAGGTCAAGG CCCAGTATGA GGAGATTGCC AACCGCAGCC GGACAGAAGC CGAGTCCTGG 1081 TATCAGACCA AGTATGAGGA GCTGCAGCAG ACAGCTGGCC GGCATGGCGA TGACCTCCGC 1141 AACACCAAGC ATGAGATCTC TGAGATGAAC CGGATGATCC AGAGGCTGAG AGCCGAGATT 1201 GACAATGTCA AGAAACAGTG CGCCAATCTG CAGAACGCCA TTGCGGATGC CGAGCAGCGT 1261 GGGGAGCTGG CCCTCAAGGA TGCCAGGAAC AAGCTGGCCG AGCTGGAGGA GGCCCTGCAG 1321 AAGGCCAAGC AGGACATGGC CCGGCTGCTG CGTGAGTACC AGGAGCTCAT GAACACCAAG 1381 CTGGCCCTGG ACGTGGAGAT CGCCACTTAC CGCAAGCTGC TGGAGGGCGA GGAATGCAGA 1441 CTCAGTGGAG AAGGAGTTGG ACCAGTCAAC ATCTCTGTTG TCACAAGCAG TGTTTCCTCT 1501 GGATATGGCA GTGGCAGTGG CTATGGCGGT GGCCTCGGTG GAGGTCTTGG CGGCGGCCTC 1561 GGTGGAGGTC TTGCCGGAGG TAGCAGTGGA AGCTACTACT CCAGCAGCAG TGGGGGTGTC 1621 GGCCTAGGTG GTGGGCTCAG TGTGGGGGGC TCTGGCTTCA GTGCAAGCAG TGGCCGAGGG 1681 CTGGGGGTGG GCTTTGGCAG TGGCGGGGGT AGCAGCTCCA GCGTCAAATT TGTCTCCACC 1741 ACCTCCTCCT CCCGGAAGAG CTTCAAGAGC TAA
Quality Control:cDNA sequencing results is available upon request.
