Human KRT20 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13198-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

KRT20 cDNA Clone Datasheet

KRT20 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_019010.2
cDNA Size:	1275
Gene Synonym:	K20; CD20; CK20; CK-20; KRT21; MGC35423;KRT20
Source:	Human

KRT20 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens keratin 20 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

KRT20 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KRT20 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KRT20 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGATTTCA GTCGCAGAAG CTTCCACAGA AGCCTGAGCT CCTCCTTGCA GGCCCCTGTA
61       GTCAGTACAG TGGGCATGCA GCGCCTCGGG ACGACACCCA GCGTTTATGG GGGTGCTGGA
121      GGCCGGGGCA TCCGCATCTC CAACTCCAGA CACACGGTGA ACTATGGGAG CGATCTCACA
181      GGCGGCGGGG ACCTGTTTGT TGGCAATGAG AAAATGGCCA TGCAGAACCT AAATGACCGT
241      CTAGCGAGCT ACCTAGAAAA GGTGCGGACC CTGGAGCAGT CCAACTCCAA ACTTGAAGTG
301      CAAATCAAGC AGTGGTACGA AACCAACGCC CCGAGGGCTG GTCGCGACTA CAGTGCATAT
361      TACAGACAAA TTGAAGAGCT GCGAAGTCAG ATTAAGGATG CTCAACTGCA AAATGCTCGG
421      TGTGTCCTGC AAATTGATAA TGCTAAACTG GCTGCTGAGG ACTTCAGACT GAAGTATGAG
481      ACTGAGAGAG GAATACGTCT AACAGTGGAA GCTGATCTCC AAGGCCTGAA TAAGGTCTTT
541      GATGACCTAA CCCTACATAA AACAGATTTG GAGATTCAAA TTGAAGAACT GAATAAAGAC
601      CTAGCTCTCC TCAAAAAGGA GCATCAGGAG GAAGTCGATG GCCTACACAA GCATCTGGGC
661      AACACTGTCA ATGTGGAGGT TGATGCTGCT CCAGGCCTGA ACCTTGGCGT CATCATGAAT
721      GAAATGAGGC AGAAGTATGA AGTCATGGCC CAGAAGAACC TTCAAGAGGC CAAAGAACAG
781      TTTGAGAGAC AGACTGCAGT TCTGCAGCAA CAGGTCACAG TGAATACTGA AGAATTAAAA
841      GGAACTGAGG TTCAACTAAC GGAGCTGAGA CGCACCTCCC AGAGCCTTGA GATAGAACTC
901      CAGTCCCATC TCAGCATGAA AGAGTCTTTG GAGCACACTC TAGAGGAGAC CAAGGCCCGT
961      TACAGCAGCC AGTTAGCCAA CCTCCAGTCG CTGTTGAGCT CTCTGGAGGC CCAACTGATG
1021     CAGATTCGGA GTAACATGGA ACGCCAGAAC AACGAATACC ATATCCTTCT TGACATAAAG
1081     ACTCGACTTG AACAGGAAAT TGCTACTTAC CGCCGCCTTC TGGAAGGAGA AGACGTAAAA
1141     ACTACAGAAT ATCAGTTAAG CACCCTGGAA GAGAGAGATA TAAAGAAAAC CAGGAAGATT
1201     AAGACAGTCG TGCAAGAAGT AGTGGATGGC AAGGTCGTGT CATCTGAAGT CAAAGAGGTG
1261     GAAGAAAATA TCTAA

Quality Control:cDNA sequencing results is available upon request.