Human KRT19 Gene cDNA Clone / ORF Clone

KRT19 cDNA Clone Datasheet

KRT19 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_002276.4
• cDNA Size: 1203
• Gene Synonym: K19, CK19, K1CS, MGC15366, KRT19
• Source: Human

KRT19 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens keratin 19 DNA.
• Vector: pGEM-T Vector

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for two point mutations: 169 C/G resulting in the amino acid pro substitution by ala and 90 C/T, 471 T/C not causing the amino acid variation.

KRT19 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KRT19 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KRT19 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACTTCCT ACAGCTATCG CCAGTCGTCG GCCACGTCGT CCTTCGGAGG CCTGGGCGGC
61       GGCTCCGTGC GTTTTGGGCC GGGGGTCGCT TTTCGCGCGC CCAGCATTCA CGGGGGCTCC
121      GGCGGCCGCG GCGTATCCGT GTCCTCCGCC CGCTTTGTGT CCTCGTCCTC CTCGGGGGGC
181      TACGGCGGCG GCTACGGCGG CGTCCTGACC GCGTCCGACG GGCTGCTGGC GGGCAACGAG
241      AAGCTAACCA TGCAGAACCT CAACGACCGC CTGGCCTCCT ACCTGGACAA GGTGCGCGCC
301      CTGGAGGCGG CCAACGGCGA GCTAGAGGTG AAGATCCGCG ACTGGTACCA GAAGCAGGGG
361      CCTGGGCCCT CCCGCGACTA CAGCCACTAC TACACGACCA TCCAGGACCT GCGGGACAAG
421      ATTCTTGGTG CCACCATTGA GAACTCCAGG ATTGTCCTGC AGATCGACAA CGCCCGTCTG
481      GCTGCAGATG ACTTCCGAAC CAAGTTTGAG ACGGAACAGG CTCTGCGCAT GAGCGTGGAG
541      GCCGACATCA ACGGCCTGCG CAGGGTGCTG GATGAGCTGA CCCTGGCCAG GACCGACCTG
601      GAGATGCAGA TCGAAGGCCT GAAGGAAGAG CTGGCCTACC TGAAGAAGAA CCATGAGGAG
661      GAAATCAGTA CGCTGAGGGG CCAAGTGGGA GGCCAGGTCA GTGTGGAGGT GGATTCCGCT
721      CCGGGCACCG ATCTCGCCAA GATCCTGAGT GACATGCGAA GCCAATATGA GGTCATGGCC
781      GAGCAGAACC GGAAGGATGC TGAAGCCTGG TTCACCAGCC GGACTGAAGA ATTGAACCGG
841      GAGGTCGCTG GCCACACGGA GCAGCTCCAG ATGAGCAGGT CCGAGGTTAC TGACCTGCGG
901      CGCACCCTTC AGGGTCTTGA GATTGAGCTG CAGTCACAGC TGAGCATGAA AGCTGCCTTG
961      GAAGACACAC TGGCAGAAAC GGAGGCGCGC TTTGGAGCCC AGCTGGCGCA TATCCAGGCG
1021     CTGATCAGCG GTATTGAAGC CCAGCTGGGC GATGTGCGAG CTGATAGTGA GCGGCAGAAT
1081     CAGGAGTACC AGCGGCTCAT GGACATCAAG TCGCGGCTGG AGCAGGAGAT TGCCACCTAC
1141     CGCAGCCTGC TCGAGGGACA GGAAGATCAC TACAACAATT TGTCTGCCTC CAAGGTCCTC
1201     TGA

 

Quality Control:cDNA sequencing results is available upon request.