Human KRT18 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13197-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

KRT18 cDNA Clone Datasheet

KRT18 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_000224.2
cDNA Size:	1293
Gene Synonym:	K18; CYK18;KRT18
Source:	Human

KRT18 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens keratin 18 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

KRT18 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KRT18 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KRT18 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCTTCA CCACTCGCTC CACCTTCTCC ACCAACTACC GGTCCCTGGG CTCTGTCCAG
61       GCGCCCAGCT ACGGCGCCCG GCCGGTCAGC AGCGCGGCCA GCGTCTATGC AGGCGCTGGG
121      GGCTCTGGTT CCCGGATCTC CGTGTCCCGC TCCACCAGCT TCAGGGGCGG CATGGGGTCC
181      GGGGGCCTGG CCACCGGGAT AGCCGGGGGT CTGGCAGGAA TGGGAGGCAT CCAGAACGAG
241      AAGGAGACCA TGCAAAGCCT GAACGACCGC CTGGCCTCTT ACCTGGACAG AGTGAGGAGC
301      CTGGAGACCG AGAACCGGAG GCTGGAGAGC AAAATCCGGG AGCACTTGGA GAAGAAGGGA
361      CCCCAGGTCA GAGACTGGAG CCATTACTTC AAGATCATCG AGGACCTGAG GGCTCAGATC
421      TTCGCAAATA CTGTGGACAA TGCCCGCATC GTTCTGCAGA TTGACAATGC CCGTCTTGCT
481      GCTGATGACT TTAGAGTCAA GTATGAGACA GAGCTGGCCA TGCGCCAGTC TGTGGAGAAC
541      GACATCCATG GGCTCCGCAA GGTCATTGAT GACACCAATA TCACACGACT GCAGCTGGAG
601      ACAGAGATCG AGGCTCTCAA GGAGGAGCTG CTCTTCATGA AGAAGAACCA CGAAGAGGAA
661      GTAAAAGGCC TACAAGCCCA GATTGCCAGC TCTGGGTTGA CCGTGGAGGT AGATGCCCCC
721      AAATCTCAGG ACCTCGCCAA GATCATGGCA GACATCCGGG CCCAATATGA CGAGCTGGCT
781      CGGAAGAACC GAGAGGAGCT AGACAAGTAC TGGTCTCAGC AGATTGAGGA GAGCACCACA
841      GTGGTCACCA CACAGTCTGC TGAGGTTGGA GCTGCTGAGA CGACGCTCAC AGAGCTGAGA
901      CGTACAGTCC AGTCCTTGGA GATCGACCTG GACTCCATGA GAAATCTGAA GGCCAGCTTG
961      GAGAACAGCC TGAGGGAGGT GGAGGCCCGC TACGCCCTAC AGATGGAGCA GCTCAACGGG
1021     ATCCTGCTGC ACCTTGAGTC AGAGCTGGCA CAGACCCGGG CAGAGGGACA GCGCCAGGCC
1081     CAGGAGTATG AGGCCCTGCT GAACATCAAG GTCAAGCTGG AGGCTGAGAT CGCCACCTAC
1141     CGCCGCCTGC TGGAAGATGG CGAGGACTTT AATCTTGGTG ATGCCTTGGA CAGCAGCAAC
1201     TCCATGCAAA CCATCCAAAA GACCACCACC CGCCGGATAG TGGATGGCAA AGTGGTGTCT
1261     GAGACCAATG ACACCAAAGT TCTGAGGCAT TAA

Quality Control:cDNA sequencing results is available upon request.