Human KRT15 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13210-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

KRT15 cDNA Clone Datasheet

KRT15 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_002275.3
cDNA Size:	1371
Gene Synonym:	K15; CK15; K1CO; KRT15
Source:	Human

KRT15 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens keratin 15 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 439 A/G resulting in the amino acid Thr substitution by Ala.

KRT15 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KRT15 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KRT15 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGACCACCA CATTTCTGCA AACTTCTTCC TCCACCTTTG GGGGTGGCTC AACCCGAGGG
61       GGTTCCCTCC TGGCTGGGGG AGGTGGCTTT GGTGGGGGGA GTCTCTCTGG GGGAGGTGGA
121      AGCCGAAGTA TCTCAGCTTC TTCTGCTAGG TTTGTCTCTT CAGGGTCAGG AGGAGGATAT
181      GGGGGTGGCA TGAGGGTCTG TGGCTTTGGT GGAGGGGCTG GTAGTGTTTT CGGTGGAGGC
241      TTTGGAGGGG GCGTTGGTGG GGGTTTTGGT GGTGGCTTTG GTGGTGGCGA TGGTGGTCTC
301      CTCTCTGGCA ATGAGAAAAT TACCATGCAG AACCTCAATG ACCGCCTGGC CTCCTACCTG
361      GACAAGGTAC GTGCCCTGGA GGAGGCCAAT GCTGACCTGG AGGTGAAGAT CCATGACTGG
421      TACCAGAAGC AGACCCCAGC CAGCCCAGAA TGCGACTACA GCCAATACTT CAAGACCATT
481      GAAGAGCTCC GGGACAAGAT CATGGCCACC ACCATCGACA ACTCCCGGGT CATCCTGGAG
541      ATCGACAATG CCAGGCTGGC TGCGGACGAC TTCAGGCTCA AGTATGAGAA TGAGCTGGCC
601      CTGCGCCAGG GCGTTGAGGC TGACATCAAC GGCTTGCGCC GAGTCCTGGA TGAGCTGACC
661      CTGGCCAGGA CTGACCTGGA GATGCAGATC GAGGGCCTGA ATGAGGAGCT AGCCTACCTG
721      AAGAAGAACC ACGAAGAGGA GATGAAGGAG TTCAGCAGCC AGCTGGCCGG CCAGGTCAAT
781      GTGGAGATGG ACGCAGCACC GGGTGTGGAC CTGACCCGTG TGCTGGCAGA GATGAGGGAG
841      CAGTACGAGG CCATGGCGGA GAAGAACCGC CGGGATGTCG AGGCCTGGTT CTTCAGCAAG
901      ACTGAGGAGC TGAACAAAGA GGTGGCCTCC AACACAGAAA TGATCCAGAC CAGCAAGACG
961      GAGATCACAG ACCTGAGACG CACGATGCAG GAGCTGGAGA TCGAGCTGCA GTCCCAGCTC
1021     AGCATGAAAG CTGGGCTGGA GAACTCACTG GCCGAGACAG AGTGCCGCTA TGCCACGCAG
1081     CTGCAGCAGA TCCAGGGGCT CATTGGTGGC CTGGAGGCCC AGCTGAGTGA GCTCCGATGC
1141     GAGATGGAGG CTCAGAACCA GGAGTACAAG ATGCTGCTTG ACATAAAGAC ACGGCTGGAG
1201     CAGGAGATCG CTACTTACCG CAGCCTGCTC GAGGGCCAGG ATGCCAAGAT GGCTGGCATT
1261     GGCATCAGGG AAGCCTCTTC AGGAGGTGGT GGTAGCAGCA GCAATTTCCA CATCAATGTA
1321     GAAGAGTCAG TGGATGGACA GGTGGTTTCT TCCCACAAGA GAGAAATCTA A

Quality Control:cDNA sequencing results is available upon request.