Human KRT13 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
HG13234-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

KRT13 cDNA Clone Datasheet

KRT13 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_002274.3
cDNA Size:	1249
Gene Synonym:	K13; CK13; MGC3781; MGC161462; KRT13
Source:	Human

KRT13 cDNA Clone Description

cDNA Description:	ORF Clone of Homo sapiens keratin 13 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Same as Gene Bank Ref. ID sequence with the nucleotide 1247-1260 deletion mutation, and the point mutation 1261-1263 TAG/TGA not causing the amino acid variation.

KRT13 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KRT13 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KRT13 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCCTCC GCCTGCAGAG CTCCTCTGCC AGCTATGGAG GTGGTTTCGG GGGTGGCTCT
61       TGCCAGCTGG GAGGAGGCCG TGGTGTCTCT ACCTGTTCAA CTCGGTTTGT GTCTGGGGGA
121      TCAGCTGGGG GCTATGGAGG CGGCGTGAGC TGTGGTTTTG GTGGAGGGGC TGGTAGTGGC
181      TTTGGAGGTG GCTATGGAGG TGGCCTTGGA GGTGGCTATG GAGGTGGCCT TGGAGGTGGC
241      TTTGGTGGGG GTTTTGCTGG TGGCTTTGTT GACTTTGGTG CTTGTGATGG CGGCCTCCTC
301      ACTGGCAATG AGAAGATCAC CATGCAGAAC CTCAACGACC GCCTGGCTTC CTACCTGGAG
361      AAGGTGCGCG CCCTGGAGGA GGCCAACGCT GACCTGGAGG TGAAGATCCG TGACTGGCAC
421      CTGAAGCAGA GCCCAGCTAG CCCTGAGCGG GACTACAGCC CCTACTACAA GACCATTGAA
481      GAGCTCCGGG ACAAGATCCT GACCGCCACC ATTGAAAACA ACCGGGTCAT CCTGGAGATT
541      GACAATGCCA GGCTGGCTGC GGACGACTTC AGGCTCAAGT ATGAGAATGA GCTGGCCCTG
601      CGCCAGAGCG TGGAGGCCGA CATCAACGGC CTGCGCCGGG TGCTGGATGA GCTCACTCTG
661      TCTAAGACTG ACCTGGAGAT GCAGATCGAG AGCCTGAATG AAGAGCTAGC CTACATGAAG
721      AAGAACCATG AAGAGGAGAT GAAGGAATTT AGCAACCAGG TGGTCGGCCA GGTCAACGTG
781      GAGATGGATG CCACCCCAGG CATTGACCTG ACCCGCGTGC TGGCAGAGAT GAGGGAGCAG
841      TACGAGGCCA TGGCAGAGAG GAACCGCCGG GATGCTGAGG AATGGTTCCA CGCCAAGAGT
901      GCAGAGCTGA ACAAGGAGGT GTCTACCAAC ACTGCCATGA TTCAGACCAG CAAGACAGAG
961      ATCACGGAGC TCAGGCGCAC GCTCCAAGGC CTGGAGATTG AGCTGCAGTC CCAGCTGAGC
1021     ATGAAAGCGG GGCTGGAGAA CACGGTGGCA GAGACGGAGT GCCGCTATGC CCTGCAGCTG
1081     CAGCAGATCC AGGGACTCAT CAGCAGCATC GAGGCCCAGC TGAGCGAGCT CCGCAGTGAG
1141     ATGGAGTGCC AGAACCAAGA GTACAAGATG CTGCTGGACA TCAAGACACG TCTGGAGCAG
1201     GAGATCGCCA CCTACCGCAG CCTGCTCGAG GGCCAGGACG CCAAGATGA

Quality Control:cDNA sequencing results is available upon request.