Rat KLRK1 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80250-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

KLRK1 cDNA Clone Datasheet

KLRK1 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_133512.1
cDNA Size:	648
Gene Synonym:	NKG2D; Nkrp2;Klrk1
Source:	Rat

KLRK1 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref.ID sequence.

KLRK1 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KLRK1 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KLRK1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGCAAAT GCCATAATTA TGACCTCAAG CCAGCAAAGT GGGATACTTC TCAAGAGCAT
61       CAGAAACAAA GATCTGCACT CCCTACCAGC CGACCTGGAG AGAATGGTAT CATAAGACGA
121      AGGTCTTCTA TAGAAGAACT CAAAATATCT CCACTGTTCG TTGTTCGAGT CCTTGTTGCA
181      GCCATGACCA TTCGCTTCAC CGTTATCACA TTGACATGGC TTGCTGTTTT CATAACTTTG
241      TTATGCAACA AGGAAGTTTC AGTTTCCTCA AGAGAGGGCT ACTGTGGCCC ATGCCCTAAC
301      GACTGGATAT GTCACAGAAA CAACTGTTAC CAATTTTTTA ATGAGAACAA AGCCTGGAAC
361      CAGAGCCAAG CTTCCTGTTT GTCTCAAAAT TCCAGCCTGC TGAAGATATA CAGTAAAGAA
421      GAACAGGATT TCTTAAAGCT GGTTAAGTCC TATCACTGGA TGGGACTAGT CCAGAGCCCA
481      GCAAATGGCT CCTGGCAGTG GGAAGATGGC TCCTCTCTAT CACCCAACGA GTTAACACTG
541      GTGAAAACAC CAAGCGGAAC CTGTGCTGTC TATGGCTCAA GCTTTAAGGC CTACACAGAA
601      GACTGTTCAA ATCCAAACAC ATACATCTGT ATGAAAAGGG CAGTGTAA

Quality Control:cDNA sequencing results is available upon request.