|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus killer cell lectin-like receptor subfamily K, member 1 DNA.|
|NKG2D, Nkrp2, Klrk1|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat KLRK1 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80250-G-F|
|Rat KLRK1 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80250-G-H|
|Rat KLRK1 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80250-G-M|
|Rat KLRK1 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80250-G-N|
|Rat KLRK1 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80250-G-Y|
|Product name||Product name|
NKG2D, also known as CD314, is an immune receptor which consists of two disulphide-linked type II transmembrane proteins with short intracellular proteins uncapable to transduce signals. In order to transduce signals, NKG2D needs adaptor proteins and it uses two adaptor proteins, DAP10 and DAP12. These two adaptor proteins associate as homodimers to NKG2D- therefore the entire receptor complex appears as a hexamer. NKG2D can send co-stimulatory signals to activate CD8 T cells. NKG2D also plays an important role in viral control. Cellular stress can induce ligands for NKG2D which results in the cell susceptible to NK cell-mediated lysis.