Cynomolgus monkey KLRC3 Gene cDNA Clone / ORF Clone

KLRC3 cDNA Clone Datasheet

KLRC3 cDNA Clone Product Information

• cDNA Size: 681
• Gene Synonym: LOC709790;KLRC2; NKG2C
• Source: Cynomolgus monkey

KLRC3 cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) killer cell lectin-like receptor subfamily C, member 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 272-274 GTG/ATA, 314 A/T resulting in the amino acid Val substitution by Ile, Asn substitution by Ile and 675 T/C not causing the amino acid variation.
  Please check the sequence information before order.

KLRC3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KLRC3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KLRC3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATAAAC AAAGAGGAAC CTTCTCAGAA GTGAGTCTGG CCCAGGATCC AAAGAGGCAG
61       CAAAGGAAAC CTAAAGGCAA TAAAAGCTCC ATTTCAGGAA CCGAACAGGA AATATTCCAA
121      GTAGAATTAA ACCTTCAAAA TCCTTCTCTG AATCATCAAG GGATTGATCA AATATATGAC
181      TGCCAAGGTT TACTGCCACC TCCAGAGAAG CTCACTGCTG AGGTCCTGGG AATCATTTGC
241      ATTGTCCTGA TGGCCACTGT GTTAAAAACA ATAGTTCTTA TTCCTTTCCT GGAGCAGAAC
301      AATTCTTTCC CGATTACAAG AACCCAGAAA GTACGTCACT GTGGCCATTG TCCTGAGGAG
361      TGGATTACAT ATTCCAACAG TTGTTATTAC ATTGGCAAGG AAAAAAGAAC TTGGGCAGAG
421      AGTTTGCTGG CCTGTACTTC AAAGAACTCT AGTCTGCTTT CTATAGATAA TGAAGAAGAA
481      ATGAAATTTC TGACTGCCAT TTCACCATCC ACATGGACTG GTGTGTTTCG TGACAGCAGT
541      CATCATCCAT GGGTGACAAT CAACGGTCTG ACTTTCAAAC ATGAGATAAA AGACTCAGAT
601      CATGCTGAAT ATAACTGTGC AATGCTACAT CTAGATAGAC TTAAATCAGT CCAGTGTGGA
661      TCTTCAAAAA GATACTATTA G

 

Quality Control:cDNA sequencing results is available upon request.