Human KLKB1 Gene cDNA Clone / ORF Clone

Catalog	Size (Price)	Quantity	In Stock	Operation	Other Information
HG10311-M	10µg ($95)		YES	Change Vector

General Information

Manufactured By: Sino Biological Inc.

Gene Bank Ref.ID:   NM_000892.3

cDNA Size:   1917

Synonym:  KLK3

Source:  Human.

Description

cDNA Description:  ORF Clone of Homo sapiens kallikrein B, plasma (Fletcher factor) 1 DNA.

Vector:  Vector pMD18-T Simple

Sequence Description:  Identical with the Gene Bank Ref. ID sequence except for two point mutations: 428 G/A causing the 143 Ser to be replaced by Asn, and 1761 T/C not causing any amino acid variation.

Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

Usage Protocol

Remove the marked edge in the center of the spot .
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O . Ensure the filter paper is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to a heating block at 95℃ for 15–30 min .
At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA .

cDNA Sequence and Quality Control

Sequence:

1        ATGATTTTAT TCAAGCAAGC AACTTATTTC ATTTCCTTGT TTGCTACAGT TTCCTGTGGA
61       TGTCTGACTC AACTCTATGA AAACGCCTTC TTCAGAGGTG GGGATGTAGC TTCCATGTAC
121      ACCCCAAATG CCCAATACTG CCAGATGAGG TGCACATTCC ACCCAAGGTG TTTGCTATTC
181      AGTTTTCTTC CAGCAAGTTC AATCAATGAC ATGGAGAAAA GGTTTGGTTG CTTCTTGAAA
241      GATAGTGTTA CAGGAACCCT GCCAAAAGTA CATCGAACAG GTGCAGTTTC TGGACATTCC
301      TTGAAGCAAT GTGGTCATCA AATAAGTGCT TGCCATCGAG ACATTTATAA AGGAGTTGAT
361      ATGAGAGGAG TCAATTTTAA TGTGTCTAAG GTTAGCAGTG TTGAAGAATG CCAAAAAAGG
421      TGCACCAATA ACATTCGCTG CCAGTTTTTT TCATATGCCA CGCAAACATT TCACAAGGCA
481      GAGTACCGGA ACAATTGCCT ATTAAAGTAC AGTCCCGGAG GAACACCTAC CGCTATAAAG
541      GTGCTGAGTA ACGTGGAATC TGGATTCTCA CTGAAGCCCT GTGCCCTTTC AGAAATTGGT
601      TGCCACATGA ACATCTTCCA GCATCTTGCG TTCTCAGATG TGGATGTTGC CAGGGTTCTC
661      ACTCCAGATG CTTTTGTGTG TCGGACCATC TGCACCTATC ACCCCAACTG CCTCTTCTTT
721      ACATTCTATA CAAATGTATG GAAAATCGAG TCACAAAGAA ATGTTTGTCT TCTTAAAACA
781      TCTGAAAGTG GCACACCAAG TTCCTCTACT CCTCAAGAAA ACACCATATC TGGATATAGC
841      CTTTTAACCT GCAAAAGAAC TTTACCTGAA CCCTGCCATT CTAAAATTTA CCCGGGAGTT
901      GACTTTGGAG GAGAAGAATT GAATGTGACT TTTGTTAAAG GAGTGAATGT TTGCCAAGAG
961      ACTTGCACAA AGATGATTCG CTGTCAGTTT TTCACTTATT CTTTACTCCC AGAAGACTGT
1021     AAGGAAGAGA AGTGTAAGTG TTTCTTAAGA TTATCTATGG ATGGTTCTCC AACTAGGATT
1081     GCGTATGGGA CACAAGGGAG CTCTGGTTAC TCTTTGAGAT TGTGTAACAC TGGGGACAAC
1141     TCTGTCTGCA CAACAAAAAC AAGCACACGC ATTGTTGGAG GAACAAACTC TTCTTGGGGA
1201     GAGTGGCCCT GGCAGGTGAG CCTGCAGGTG AAGCTGACAG CTCAGAGGCA CCTGTGTGGA
1261     GGGTCACTCA TAGGACACCA GTGGGTCCTC ACTGCTGCCC ACTGCTTTGA TGGGCTTCCC
1321     CTGCAGGATG TTTGGCGCAT CTATAGTGGC ATTTTAAATC TGTCAGACAT TACAAAAGAT
1381     ACACCTTTCT CACAAATAAA AGAGATTATT ATTCACCAAA ACTATAAAGT CTCAGAAGGG
1441     AATCATGATA TCGCCTTGAT AAAACTCCAG GCTCCTTTGA ATTACACTGA ATTCCAAAAA
1501     CCAATATGCC TACCTTCCAA AGGTGACACA AGCACAATTT ATACCAACTG TTGGGTAACC
1561     GGATGGGGCT TCTCGAAGGA GAAAGGTGAA ATCCAAAATA TTCTACAAAA GGTAAATATT
1621     CCTTTGGTAA CAAATGAAGA ATGCCAGAAA AGATATCAAG ATTATAAAAT AACCCAACGG
1681     ATGGTCTGTG CTGGCTATAA AGAAGGGGGA AAAGATGCTT GTAAGGGAGA TTCAGGTGGT
1741     CCCTTAGTTT GCAAACACAA CGGAATGTGG CGTTTGGTGG GCATCACCAG CTGGGGTGAA
1801     GGCTGTGCCC GCAGGGAGCA ACCTGGTGTC TACACCAAAG TCGCTGAGTA CATGGACTGG
1861     ATTTTAGAGA AAACACAGAG CAGTGATGGA AAAGCTCAGA TGCAGTCACC AGCATGA

Quality Control: cDNA sequencing results is available upon request.

Product Catalog

Human KLKB1 Gene cDNA Clone / ORF Clone