KLK-8 / Kallikrein-8 Protein (His Tag)

Kallikrein-8 / KLK8 Protein

Kallikrein-8 / KLK8 Protein Product Information

• Synonym: UNQ283/PRO322, HNP, NP, NRPN, PRSS19, TADG14
• Protein Construction: A DNA sequence encoding the human KLK8 isoform 1 ( O60259-1 )( Met 1 - Gly 260 ) was expressed, with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

Kallikrein-8 / KLK8 Protein QC Testing

• Purity: > 98 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to inhibit thrombin ( R&D Systems, Catalog# 1473SE ) cleavage of a fluorogenic peptide substrate Boc-VPR-AMC ( R&D Systems , Catalog# ES011 )
  The specific activity is > 500 pmoles / min / μg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 29
• Molecular Mass: The recombinant human KLK8 consists of 243 amino acids and predictes a molecular mass of 26.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKLK8 is approximately 36 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KLK8 protein

Kallikrein-8 / KLK8 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Kallikrein-8 / KLK8 Protein Description

Kallikrein-8, also known as Neuropsin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein and KLK8, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. It is a serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Kallikrein-8 / KLK8 plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway. It regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. It is involved in skin desquamation and keratinocyte proliferation and plays a role in the secondary phase of pathogenesis following spinal cord injury. It also cleaves L1CAM in response to increased neural activity. It induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Kallikrein-8 / KLK8 is expressed at high levels in serum, ascites fluid and tumor cytosol of advanced stage ovarian cancer patients and may serve as a marker of ovarian cancer. Kallikrein-8 / KLK8 may have potential clinical value for disease diagnosis or prognosis and it may also be a useful therapeutic target.

References

1. Magklara A., et al., 2001, Clin.Cancer Res. 7: 806-11.
2. Shimizu-Okabe C., et al., 2001, NeuroReport 12: 2747-51.
3. Kuwae K., et al., 2002, Mol. Pathol. 55: 235-41.
4. Kishi T., et al., 2003, Cancer Res. 63: 2771-4.
5. Scott F.L., et al., 2007, J. Biochem. 142: 435-42.