Mouse KLK-7 Gene cDNA Clone / ORF Clone

KLK7 cDNA Clone Datasheet

KLK7 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_011872.2
• cDNA Size: 750
• Gene Synonym: SCCE; Prss6; Klk7
• Source: Mouse

KLK7 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus kallikrein related-peptidase 7 (chymotryptic, stratum corneum) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

KLK7 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KLK7 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KLK7 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGAGTCT GGCTCCTTTC CCTGATAACT GTGCTGCTGT CTTTAGCCCT GGAAACCGCT
61       GGACAAGGAG AAAGGATTAT AGATGGCTAC AAATGTAAAG AAGGCTCGCA CCCATGGCAG
121      GTGGCTCTGC TCAAAGGCAA TCAGCTTCAC TGTGGAGGGG TGCTGGTGGA CAAATACTGG
181      GTGCTCACAG CAGCCCACTG CAAAATGGGT CAGTACCAAG TACAGCTGGG CAGTGATAAA
241      ATAGGGGACC AGAGTGCCCA GAAGATCAAG GCTACAAAGT CATTCCGGCA CCCTGGCTAC
301      TCCACAAAGA CCCACGTCAA TGACATCATG CTTGTGAGGT TGGATGAGCC AGTCAAGATG
361      TCATCCAAAG TGGAGGCAGT CCAGCTTCCT GAACACTGTG AACCCCCAGG GACGTCATGT
421      ACCGTCTCTG GATGGGGCAC CACAACCAGC CCAGACGTGA CCTTTCCCTC GGATCTTATG
481      TGCTCGGATG TGAAGCTCAT CTCCTCCAGG GAGTGCAAGA AGGTGTACAA GGACCTGCTG
541      GGGAAAACCA TGCTGTGTGC TGGCATTCCT GACTCTAAGA CCAACACGTG CAATGGTGAC
601      TCAGGGGGGC CCTTGGTGTG CAACGACACC CTCCAAGGTC TGGTGTCCTG GGGTACCTAC
661      CCTTGTGGCC AGCCCAACGA CCCAGGCGTC TACACTCAAG TCTGCAAGTA CAAACGGTGG
721      GTGATGGAGA CCATGAAAAC CCATCGCTAA

 

Quality Control:cDNA sequencing results is available upon request.