KLK6 Protein (His Tag)

Kallikrein-related peptidase 6 Protein Datasheet

KLK6 / Klk7 Protein Product Information

• Synonym: Bssp, Klk7, PRSS18, PRSS9, SP59, Hk6
• Protein Construction: A DNA sequence encoding the human KLK6 isoform 1 (Q92876-1) (Met 1- Lys 244) , was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

KLK6 / Klk7 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-QARAMC, R&D Systems, Catalog # ES014.
  The specific activity is > 250 pmols/min/μg.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 17
• Molecular Mass: The secreted recombinant human KLK6 (pro form) consists of 239 amino acids and has a predicted molecular mass of 26.6 kDa. The apparent molecular mass of rhKLK6 is approximately 10&25 kDa in SDS-PAGE under reducing conditions due to autolysis.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KLK6 / Klk7 protein

KLK6 / Klk7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KLK6 / Klk7 Protein Description

KLK6 (kallikrein-related peptidase 6), also known as Klk7, contains 1 peptidase S1 domain and belongs to the peptidase S1 family, Kallikrein subfamily. In tissue culture, KLK6 has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Klk7 exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. At the tissue level, Klk7's highest concentrations found in glandular tissues such as salivary glands followed by lung, colon, fallopian tube, placenta, breast, pituitary and kidney. KLK6 is overexpressed in primary breast tumors but not expressed in metastatic tumors. KLK6 may be involved in the pathogenesis of Parkinson disease and other synucleinopathies because it shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Klk7 also may be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis.

References

1. Krenzer S. et al., 2011, J Invest Dermatol. 131 (11): 2281-8.
2. Nathalie HV. et al., 2009, J Cell Mol Med. 13 (9B): 4014-22.
3. Kim JT. et al., 2011, Cancer. 117 (12): 2608-19.
4. Scarisbrick IA. et al., 2011, PLoS One. 6 (3): e18376.