KLK-4 / Kallikrein-4 Protein (His Tag)

Kallikrein-Like Protein 1 ( KLK4 / Kallikrein-4 ) Protein

KLK4 / Kallikrein-4 Protein Product Information

• Synonym: AI2A1, ARM1, EMSP, EMSP1, KLK-L1, MGC116827, MGC116828, PRSS17, PSTS
• Protein Construction: A DNA sequence encoding the human KLK4 ( NP_004908.3 ) ( Met 1 - Ser 254 ) was expressed, with a polyhistidine tag at the C-terminus
• Source: Human
• Expression Host: Human Cells

KLK4 / Kallikrein-4 Protein QC Testing

• Purity: > 94 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. ( R&D Systems, Catalog # ES011 )
  The specific activity is > 250 pmoles / min / µg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 27
• Molecular Mass: The secreted recombinant human KLK4 consists of 239 amino acids and predictes a molecular mass of 25.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhKLK4 is approximately 30-35 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KLK4 protein

KLK4 / Kallikrein-4 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KLK4 / Kallikrein-4 Protein Description

Kallikrein-4, also known as Enamel matrix serine proteinase 1, Kallikrein-like protein 1, KLK-L1, Serine protease 17, KLK4, PRSS17 and EMSP1, is a secreted protein which belongs to the peptidase S1 family and Kallikrein subfamily. Kallikrein-4 / KLK4 is a serine protease expressed during enamel maturation, and proteolytic processing of the enamel matrix by KLK4 is critical for proper enamel formation. Kallikrein-4 / KLK4 contains one peptidase S1 domain. Kallikrein-4 / KLK4 is secreted by transition- and maturation-stage ameloblasts. KLK4 aggressively degrades the retained organic matrix following the termination of enamel protein secretion. Two proteases are secreted into the enamel matrix of developing teeth. The early protease is enamelysin (MMP-20). The late protease is kallikrein 4 (KLK4). The principle functions of MMP-20 and KLK4 in dental enamel formation are to facilitate the orderly replacement of organic matrix with mineral, generating an enamel layer that is harder, less porous, and unstained by retained enamel proteins. Defects in Kallikrein-4 / KLK4 are the cause of amelogenesis imperfecta hypomaturation type 2A1 (AI2A1) which is an autosomal recessive defect of enamel formation. The disorder involves both primary and secondary dentitions.

References

1. Yousef G.M. et al., 1999, Cancer Res. 59:4252-4256.
2. Hart P.S. et al., 2004, J. Med. Genet. 41:545-549.
3. Lu,Y. et al., 2008, Biol Chem. 389 (6):695-700.
4. Tye,C.E. et al., 2009, J Dent Res. 88 (4):323-7.