KLK3 / PSA / Kallikrein-3 Protein (His Tag)

Prostate-specific Antigen / Gamma-seminoprotein Protein

Kallikrein-3 / KLK-3 Protein Product Information

• Synonym: APS, KLK2A1, PSA, Hk3
• Protein Construction: A DNA sequence encoding the human KLK3 ( P07288 ) ( Met 1 - Pro 261 ) was fused with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

Kallikrein-3 / KLK-3 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide(Suc-RPY-pNA)
  The specific activity is >100 pmoles/min/μg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ala 18
• Molecular Mass: The recombinant human KLK3 consists of 255 amino acids and has a predicted molecular mass of 28.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh KLK3 is approximately 35 kDa
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KLK-3 protein

Kallikrein-3 / KLK-3 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Kallikrein-3 / KLK-3 Protein Description

Prostate-specific antigen, also known as Gamma-seminoprotein, Kallikrein-3, kallikrein-related peptidase 3, Semenogelase, KLK3 and PSA, is a secreted protein which belongs to thepeptidase S1 family and Kallikrein subfamily. KLK3/Kallikrein 3 contains one peptidase S1 domain. KLK3/Kallikrein 3 is a glycoprotein produced almost exclusively by the prostate gland. KLK3/Kallikrein 3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.It is also believed to be instrumental in dissolving the cervical mucous, allowing the entry of sperm. Human KLK3/Kallikrein 3 and human KLK2 are closely related products of the human kallikrein genes KLK3 and KLK2, respectively. Both KLK3/Kallikrein 3 and human kallikrein 2 are produced and secreted in the prostate and have important applications in the diagnosis of prostate cancer. Understanding the mechanism by which genetic variation in KLK3/Kallikrein 3 affects prostate cancer risk has important implications for study of the biological role of KLK3/Kallikrein 3 in prostate tumorigenesis.

References

1. Koistinen,H. et al., 2008,Biol Chem. 389 (6):633-42.
2. David,A. et al.,  2002,J Biol Chem. 277 (20):18084-90.
3. Andriole GL, et al., 2009, New Eng. J. Med. 360 (13): 1310.
4. Schröder FH, et al., 2009, New Eng. J. Med. 360 (13): 1320.