KLK3 / Kallikrein 3 Antibody

KLK3 / Kallikrein 3 Antibody Datasheet

KLK3 / Kallikrein 3 Antibody Product Information

• Immunogen: Recombinant Human KLK3 / Kallikrein 3 protein (Catalog#10771-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 007
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human KLK3 / Kallikrein 3 (rh KLK3 / Kallikrein 3; Catalog#10771-H08H; P07288; Met 1-Pro 261).

KLK3 / Kallikrein 3 Antibody Usage Guide

• Specificity: Human KLK3 / Kallikrein 3
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KLK3. The detection limit for Human KLK3 is approximately 0.0195 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KLK3 / Kallikrein 3 Antibody Background

Prostate-specific antigen, also known as Gamma-seminoprotein, Kallikrein-3, kallikrein-related peptidase 3, Semenogelase, KLK3 and PSA, is a secreted protein which belongs to thepeptidase S1 family and Kallikrein subfamily. KLK3/Kallikrein 3 contains one peptidase S1 domain. KLK3/Kallikrein 3 is a glycoprotein produced almost exclusively by the prostate gland. KLK3/Kallikrein 3 is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.It is also believed to be instrumental in dissolving the cervical mucous, allowing the entry of sperm. Human KLK3/Kallikrein 3 and human KLK2 are closely related products of the human kallikrein genes KLK3 and KLK2, respectively. Both KLK3/Kallikrein 3 and human kallikrein 2 are produced and secreted in the prostate and have important applications in the diagnosis of prostate cancer. Understanding the mechanism by which genetic variation in KLK3/Kallikrein 3 affects prostate cancer risk has important implications for study of the biological role of KLK3/Kallikrein 3 in prostate tumorigenesis.

References

1. Koistinen,H. et al., 2008, Biol Chem. 389 (6): 633-42.
2. David,A. et al.,2002, J Biol Chem. 277 (20): 18084-90.
3. Andriole GL, et al., 2009, New Eng. J. Med. 360 (13): 1310.
4. Schroder FH, et al., 2009, New Eng. J. Med. 360 (13): 1320.