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> ELISA > ELISA Pair Set > KLK13 / Kallikrein 13 ELISA Pair Set, Human ELISA Pair Set KLK13 / Kallikrein 13 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10199 |
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Human KLK13 / Kallikrein 13 ELISA Pair Set
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Order or Inquire for KLK13 / Kallikrein 13 ELISA Pair Set |
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High affinity Human KLK13 / Kallikrein 13 ELISA Pair Set |
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Detection limit - 39.0625 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human KLK13 / Kallikrein 13 ELISA Pair Set Related Products & Topics
Related Areas:
Enzyme>>Protease & Regulator>>Serine Protease & Regulator>>Kallikrein>>KLK13/Kallikrein 13
Cancer>>Angiogenesis>>Kallikrein>>KLK13/Kallikrein 13
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| KLK13/Kallikrein 13 | Human | KLK13/Kallikrein 13 Protein, Recombinant |
10199-H08H |
| KLK13/Kallikrein 13 | Human | KLK13/Kallikrein 13 Protein, Recombinant | 10199-HCCH |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Human KLK13/Kallikrein 13 |
ELISA | Mouse Monoclonal Antibody | 10199-MM01 |
| Human KLK13/Kallikrein 13 |
WB, ELISA | KLK13/Kallikrein 13 Antibody, Rabbit PAb | 10199-RP01 |
| Human KLK13/Kallikrein 13 |
WB, ELISA | KLK13/Kallikrein 13 Antibody, Rabbit PAb (Antigen Affinity Purified) | 10199-RP02 |
Background
Human tissue kallikrein 13 ( hK13 ), also known as KLK-L4 (kallikrein-like gene 4) and KLK13 is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.
