KLK13 / Kallikrein-13 Antibody (Antigen Affinity Purified)

KLK13 / Kallikrein-13 Antibody ( Antigen Affinity Purified )

KLK13 / Kallikrein-13 Antibody Product Information

• Immunogen: Recombinant human KLK13 protein ( Catalog #10199-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human KLK13 / Kallikrein13 ( rh KLK13; Catalog#10199-H08H; NP_056411.1; Met 1- Ile 262 ). KLK13 specific IgG was purified by human KLK13 affinity chromatography

KLK13 / Kallikrein-13 Antibody Usage Guide

• Specificity: Human KLK13 / Kallikrein-13
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KLK13 in WB. Using a DAB detection system, the detection limit for Human KLK13 is approximately 1 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human KLK13. The detection limit for human KLK13 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KLK13 / Kallikrein-13 Antibody Background

Human tissue kallikrein 13 ( hK13 ), also known as KLK-L4 (kallikrein-like gene 4) and KLK13 is a member of the human tissue kallikrein family of serine proteases having diverse physiological functions in many tissues. The KLK13 gene resides on chromosome 19q13.3-4 along with other 14 members in a gene cluster and shares a high degree of homology. KLK13 is a trypsin-like, secreted serine protease expressed specifically in the testicular tissue including prostate, salivary gland, breast, and testis. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and may play a role in metastasis. KLK13 may be involved in the pathogenesis and/or progression of breast and ovary cancers, and is regarded as a novel cancer biomarker. In addition, KLK13 interacts and forms complexes with several serum protease inhibitors, such as alpha2-macroglobulin, and its expression is regulated by steroid hormones.

References

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