KLK11 / Kallikrein-11 Protein (His Tag)

Kallikrein-related Peptidase 11 ( Kallikrein 11 / KLK11 ) Protein

Kallikrein 11 / KLK11 Protein Product Information

• Kallikrein 11: MGC33060, PRSS20, TLSP,KLK11
• Protein Construction: A DNA sequence encoding the human KLK11 isoform 1 ( NP_006844.1 ) ( Met 1 - Asn 250 ) with a C-terminal polyhistidine tag was expressed.
• Source: Human
• Expression Host: Human Cells

Kallikrein 11 / KLK11 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave a colorimetric peptide substrate D-Val-Leu-Lys-ThioBenzyl ester ( VLK-SBzl ), in the presence of 5,5’Dithio-bis ( 2-nitrobenzoic acid ) ( DTNB ). ( Edwards, K.M. et al.,1999, J. Biol. Chem. 274: 30468 )
  The specific activity is > 200 pmoles / min / µg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 19
• Molecular Mass: The secreted recombinant human KLKL11 comprises 243 amino acids with a predicted molecular mass of 27 kDa. As a result of glycosylation, rhKLK11 migrates as an approximately 40 kDa band in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KLK11 protein

Kallikrein 11 / KLK11 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Kallikrein 11 / KLK11 Protein Description

kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which is implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate.

References

1. Yoshida, S. et al., 1998, Biochim. Biophys. Acta 1399: 225-228.
2. Yousef, G.M. et al., 2000,Genomics 63: 88-96.
3. Mitsui, S. et al., 2000, Biochem. Biophys. Res. Commun. 272: 205-211.
4. Gan, L. et al., 2000, Gene 257:119-130.
5. Yousef, G.M. et al., 2001, Endocrine Rev. 22:184-204.
6. Kong, W.J. et al., 2006, J Mol Med. Jan. 84 (1): 29-36.