|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-KLK11 rabbit monoclonal antibody at 1:500 dilution
Lane A: A549 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:31 kDa
Observed band size:42 kDa
KLK11 was immunoprecipitated using:
Lane A:0.5 mg Hela Whole Cell Lysate1 µL anti-KLK11 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-KLK11 rabbit monoclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 31 kDa
Observed band size: 49 kDa
kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which is implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-Kallikrein 11 Antibody||Mouse||Monoclonal||ELISA||10767-MM11|
|Anti-Kallikrein 11 Antibody||Rabbit||Monoclonal||ELISA(Cap)||10767-R101|
|Anti-Kallikrein 11 Antibody||Mouse||Monoclonal||ELISA||10767-MM05|
|Anti-Kallikrein 11 Antibody||Rabbit||Polyclonal||ELISA||10767-RP01|
|Anti-Kallikrein 11 Antibody||Rabbit||Polyclonal||WB,ELISA||10767-RP02|