Anti-Kallikrein 11 Antibody

Cat: 10767-R121
Contact us:
+86-400-890-9989
order@sinobiological.com
Distributors
All Kallikrein 11 Reagents
CRO Services
Product recommended

All Kallikrein 11 Reagents
Anti-Kallikrein 11 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-Kallikrein 11 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human Kallikrein 11
Immunogen
Recombinant Human KLK-11 protein (Catalog#10767-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human KLK11 / Kallikrein 11 (rh KLK-11; Catalog#10767-H08H; Met 1-Asn 250; NP_006844.1).
Source
Monoclonal Rabbit IgG Clone #121
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-Kallikrein 11 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-Kallikrein 11 Antibody (Rabbit Monoclonal antibody) Images
Human KLK11/Kallikrein 11 Western blot (WB) 13275

Anti-KLK11 rabbit monoclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:31 kDa

Observed band size:42 kDa

Human KLK11/Kallikrein 11 Immunoprecipitation(IP) 14043

KLK11 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

1 µL anti-KLK11 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-KLK11 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 31 kDa

Observed band size: 49 kDa

Anti-Kallikrein 11 Antibody Alternative Names
Anti-KLK11 Antibody;Anti-PRSS20 Antibody;Anti-TLSP Antibody
Kallikrein 11 Background Information

kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which is implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate.

Full Name
kallikrein-related peptidase 11
References
  • Yoshida S., et al.,(1998), cDNA cloning and expression of a novel serine protease, TLSP. Biochim. Biophys. Acta 1399:225-228.
  • Mitsui S., et al., (2000), A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate.Biochem. Biophys. Res. Commun. 272:205-211.
  • Yousef G.M., et al.,(2000), Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family.Genomics 63:88-96.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-Kallikrein 11 Antibody Mouse Monoclonal ELISA 10767-MM11
    Anti-Kallikrein 11 Antibody Rabbit Monoclonal ELISA(Cap) 10767-R101
    Anti-Kallikrein 11 Antibody Mouse Monoclonal ELISA 10767-MM05
    Anti-Kallikrein 11 Antibody Rabbit Polyclonal ELISA 10767-RP01
    Anti-Kallikrein 11 Antibody Rabbit Polyclonal WB,ELISA 10767-RP02
    info info
    添加购物车成功! 添加购物车失败!