Mouse KLK-1 Gene cDNA Clone / ORF Clone

KLK-1 cDNA Clone Datasheet

KLK-1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_010639.7
• cDNA Size: 786
• Gene Synonym: Kal; Klk6; mGk-6; Klk1b6; 0610007D04Rik; Klk1
• Source: Mouse

KLK-1 cDNA Clone Description

• cDNA Description: ORF Clone of Mus musculus kallikrein 1 DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

KLK-1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KLK-1 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KLK-1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGTTCC TGATCCTGTT CCTAGCCCTG TCCCTAGGAG GGATTGATGC TGCACCTCCT
61       GTCCAGTCTC GAATTGTTGG AGGATTTAAC TGTGAGAAGA ATTCCCAGCC CTGGCAAGTG
121      GCTGTGTACC GCTTCACCAA ATATCAATGT GGGGGTATCC TGCTGAACGC CAACTGGGTT
181      CTCACAGCTG CCCACTGCCA TAATGACAAG TACCAGGTGT GGCTGGGCAA AAACAACTTT
241      TTGGAGGATG AACCCTCTGC CCAACACCGG CTTGTCAGCA AAGCCATCCC TCACCCTGAC
301      TTCAACATGA GCCTCCTGAA TGAGCACACC CCACAACCTG AGGATGACTA CAGCAATGAC
361      CTGATGCTGC TCCGCCTCAA AAAGCCTGCT GACATCACAG ATGTTGTGAA GCCCATCGAC
421      CTGCCCACTG AGGAGCCCAA GCTGGGGAGC ACATGCCTAG CCTCAGGCTG GGGCAGCATT
481      ACACCCGTCA AATATGAATA CCCAGATGAG CTCCAGTGTG TGAACCTCAA GCTCCTGCCT
541      AATGAGGACT GTGCCAAAGC CCACATAGAG AAGGTGACAG ATGACATGTT GTGTGCAGGA
601      GATATGGATG GAGGCAAAGA CACTTGTGCG GGTGACTCAG GAGGCCCACT GATCTGTGAT
661      GGTGTTCTCC AAGGTATCAC ATCATGGGGC CCTAGCCCTT GCGGTAAACC CAATGTGCCG
721      GGTATCTACA CCAGAGTTTT AAATTTCAAC ACCTGGATAA GAGAAACTAT GGCTGAAAAT
781      GACTGA

 

Quality Control:cDNA sequencing results is available upon request.