KLK-1 / Kallikrein-1 Protein (His Tag)

Kallikrein-1 / KLK-1 Protein

KLK-1 / Kallikrein-1 Protein Product Information

• KLK1 Synonym: KLK1, KLKR, Klk6, Hk1
• Protein Construction: A DNA sequence encoding the human KLK1 ( NP_002248.1 ) precursor ( Met 1 - Ser 262 ) was expressed with a polyhistidine tag at the C-terminus.
• Source: Human
• Expression Host: Human Cells

KLK-1 / Kallikrein-1 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Bio-activity: Measured by its ability to cleave a flourogenic peptide substrate Pro-Phe-Arg-7-amido-4-methylcoumarin ( PFR-AMC ).
  The specific activity is > 1,500 pmoles / min / µg
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 23
• Molecular Mass: The secreted recombinant human KLK1 is a monomeric protein after cleavage of the signal peptide. It consists of 251 amino acids and predictes a molecular mass of 28 kDa. In SDS-PAGE under reducing conditions, it migrates with the apparent molecular mass of 50 kDa due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KLK1 protein

KLK-1 / Kallikrein-1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KLK-1 / Kallikrein-1 Protein Description

Kallikreins are members of a highly conserved serine proteases that are involved in the post-translational modification of many polypeptides, and plays a role in diverse physiological processes. Fifteen kallikreins whose encoding genes are located in a cluster on chromosome 19 have been reported, and growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Human kallikrein 1 (KLK1), also known as tissue kallikrein, is functionally conserved in its capacity to cleave the low molecular weight kininogen to release the vasoactive peptide, Lys-bradykinin which plays a role in regulating vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Human KLK1 precursor consists of a signal peptide, a short pro peptide and a mature chain. Moreover, KLK1 was suggested to be associated with angiogensis and tumorigenesis.

References

1. Henttu, P. et al., 1990, Int. J. Cancer. 45: 654-660.
2. Clements, J. et al., 2001, Biol. Chem. 382: 5-14.
3. Bourgeois, L. et al., 1997, J. Biol. Chem. 272: 29590-29595.