KLK1 / Kallikrein 1 Antibody

KLK1 / Kallikrein 1 Antibody Datasheet

KLK1 / Kallikrein 1 Antibody Product Information

• Immunogen: Recombinant Human KLK1 / Kallikrein 1 Protein (Catalog#10407-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 105
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant Human KLK1 / Kallikrein 1 (rh KLK1 / Kallikrein 1; Catalog#10407-H08H; NP_002248.1; Met 1 - Ser 262).

KLK1 / Kallikrein 1 Antibody Usage Guide

• Specificity: Human KLK1 / Kallikrein 1
• No cross-reactivity in ELISA with

  Human KLK11

  Human KLK13

  Human KLK7

  Human HGFA

  Human F11 / Coagulation Factor XI

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human KLK1 in WB. Using a DAB detection system, the detection limit for Human KLK1 is approximately 0.125 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KLK1. The detection limit for Human KLK1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KLK1 / Kallikrein 1 Antibody Background

Kallikreins are members of a highly conserved serine proteases that are involved in the post-translational modification of many polypeptides, and plays a role in diverse physiological processes. Fifteen kallikreins whose encoding genes are located in a cluster on chromosome 19 have been reported, and growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Human kallikrein 1 (KLK1), also known as tissue kallikrein, is functionally conserved in its capacity to cleave the low molecular weight kininogen to release the vasoactive peptide, Lys-bradykinin which plays a role in regulating vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Human KLK1 precursor consists of a signal peptide, a short pro peptide and a mature chain. Moreover, KLK1 was suggested to be associated with angiogensis and tumorigenesis.

References

1. Henttu, P. et al., 1990, Int. J. Cancer. 45: 654-660.
2. Clements, J. et al., 2001, Biol. Chem. 382: 5-14.
3. Bourgeois, L. et al., 1997, J. Biol. Chem. 272: 29590-29595.