KLK-1 / Kallikrein-1 Antibody (Antigen Affinity Purified)

Kallikrein-1 / KLK-1 Antibody ( Antigen Affinity Purified )

Kallikrein-1 / KLK-1 Antibody Product Information

• Immunogen: Recombinant human KLK-1 protein ( Catalog#10407-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human KLK1 ( rh KLK-1 ; Catalog#10407-H08H ; NP_002248.1 ; Met 1 - Ser 262 ). KLK-1 specific IgG was purified by human KLK-1 affinity chromatography

Kallikrein-1 / KLK-1 Antibody Usage Guide

• Specificity: Human KLK-1 / Kallikrein-1
• Western blot: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human KLK-1 in WB. Using a DAB detection system, the detection limit for human KLK-1 is approximately 2 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human KLK-1. The detection limit for human KLK-1 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

Kallikrein-1 / KLK-1 Antibody Background

Kallikreins are members of a highly conserved serine proteases that are involved in the post-translational modification of many polypeptides, and plays a role in diverse physiological processes. Fifteen kallikreins whose encoding genes are located in a cluster on chromosome 19 have been reported, and growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. Human kallikrein 1 (KLK1), also known as tissue kallikrein, is functionally conserved in its capacity to cleave the low molecular weight kininogen to release the vasoactive peptide, Lys-bradykinin which plays a role in regulating vasodilation, blood pressure reduction, smooth muscle relaxation and contraction, pain induction and inflammation. Human KLK1 precursor consists of a signal peptide, a short pro peptide and a mature chain. Moreover, KLK1 was suggested to be associated with angiogensis and tumorigenesis.

References

1. Henttu, P. et al., 1990, Int. J. Cancer. 45: 654-660.
2. Clements, J. et al., 2001, Biol. Chem. 382: 5-14.
3. Bourgeois, L. et al., 1997, J. Biol. Chem. 272: 29590-29595.