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KLH / Keyhole Limpet Hemocyanin Antibody, Mouse MAb

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KLHAntibody Product Information
Antigen:Recombinant KLH protein
Clone ID:15F7E4G3
Ig Type:Mouse IgG1
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant KLH (Keyhole Limpet Hemocyanin). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
KLHAntibody Usage Guide
Specificity: KLH or KLH conjucted protein
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA.
Application:WB, ELISA

WB: 1-2 μg/mL

This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect KLH in WB.

ELISA: 0.5-1 μg/mL

This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect KLH or KLH conjucted protein. The detection limit for KLH is approximately 0.078 ng/well .

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"