- EGFR Signaling Pathway
- TGF-beta Signaling
- Canonical Wnt Signaling
- non-Canonical Wnt Signaling
- Notch Signaling
- p53 Pathway
- NF-kB Pathway
- Cytokine Signaling
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Keyhole Limpet Hemocyanin / KLH Antibody
|Order or Inquire for KLH Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.078 ng/well in ELISA|
Keyhole Limpet Hemocyanin / KLH Antibody Product Information
Recombinant KLH protein
|Antibody Type :||Mouse Monoclonal Antibody ( Mouse mAb Service Platform )|
Clone ID :
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS, 5% trehalose may be added in some batches. Please read the hardcopy of COA or contact our customer service to confirm the formulation.|
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant KLH ( Keyhole Limpet Hemocyanin ). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Keyhole Limpet Hemocyanin / KLH Antibody Usage Guide
KLH or KLH conjucted protein
|No cross-reactivity with human cell lysate ( 293 cell line ) in WB and ELISA.|
|Western blot :||This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect KLH in WB.|
|Direct ELISA :||This antibody can be used at 0.5 - 1 μg/mL with the appropriate secondary reagents to detect KLH or KLH conjucted protein. The detection limit for KLH is approximately 0.078 ng/well|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Keyhole Limpet Hemocyanin / KLH Antibody Related Products & Topics
Keyhole Limpet Hemocyanin / KLH Antibody Related Areas:
Keyhole Limpet Hemocyanin / KLH Antibody Background
KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory. A high quality KLH preparation with the clear opalescent blue color is the best indicator of KLH solubility. KLH is being tested as a therapeutic vaccine for a variety of cancers, including non-Hodgkins lymphoma, cutaneous melanoma, breast and bladder cancer. These vaccines use specific tumor-associated antigens (Haptens) conjugated to KLH to stimulate the body’s immune system to generate anti-tumor immune responses which can destroy tumor cells.
- Lamm, D.L. et al., 1993, Urol Res. 21 (1): 33-7.
- Lamm, D.L. et al., 1993, J Urol. 149 (3): 648-52.
- Jennemann, R. et al.,1994, J Biochem. 115 (6): 1047-52.
- Harris, J.R. et al., 1999, Micron. 30 (6): 597-623.