Cynomolgus monkey KIT Gene cDNA Clone / ORF Clone

KIT cDNA Clone Datasheet

KIT cDNA Clone Product Information

• cDNA Size: 2919
• Gene Synonym: KIT
• Source: Cynomolgus monkey

KIT cDNA Clone Description

• cDNA Description: ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Same as Gene Bank Ref. ID sequence with the nucleotide 1525-1536 deletion mutation and the point mutation 1063 T/G, 1149 A/G, 2484 T/C, 2685 G/A not causing the amino acid variation.
  Please check the sequence information before order.

KIT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KIT cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KIT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAGGCG CTCGCGGCGC CTGGGATTTT CTCTGCGTTC TGCTCTTCCT GCTCCACGTC
61       CAGACAGGCT CTTCTCAACC ATCTGTGAGT CCAGGGGAAC CGTCTCCACC ATCCATCCAT
121      CCAGCAAAAT CAGAGTTAAT AGTCCGAGTG GGCAACGAGA TTAGGCTGTT GTGCATTGAT
181      CCAGGCTTTG TCAAATGGAC TTTTGAGATC CTGGATGAAA CGAATGAGAA TAAGCAGAAT
241      GAATGGATCA CAGAAAAGGC AGAGGCCACC AACACCGGCA AATACACGTG CACCAACAAA
301      CACGGCTTAA GCAGTTCCAT TTATGTGTTT GTTAGAGATC CTGCCAAGCT TTTCCTTGTT
361      GACCGCTCCT TGTATGGGAA AGAAGACAAT GACACACTGG TCCGCTGTCC TCTCACAGAC
421      CCAGAAGTGA CCAGTTATTC CCTCAAGGGG TGCCAGGGGA AGCCTCTTCC CAAGGACTTG
481      AGGTTTGTTC CTGACCCCAA GGCTGGCATC ACGATCAAAA GCGTGAAACG CGCCTACCAT
541      CGGCTCTGTC TGCATTGTTC CGCGGACCAG GAGGGCAAGT CAGTGCTGTC GGACAAGTTC
601      ATCCTGAAAG TGAGGCCAGC CTTCAAAGCT GTGCCTGTTG TGTCTGTTTC CAAAGCAAGC
661      TATCTTCTTA GGGAAGGGGA AGAATTCACA GTGACATGCA CAATAAAAGA TGTGTCTAGT
721      TCTGTGTACT CAACGTGGAA AAGAGAAAAC AGTCAGACTA AACTACAGGA GAAATATAAT
781      AGCTGGCATC ACGGTGACTT CAATTATGAA CGTCAGGCAA CGTTGACTAT CAGTTCAGCG
841      AGAGTTAATG ATTCTGGAGT GTTCATGTGT TATGCCAATA ATACTTTTGG ATCAGCAAAT
901      GTCACAACAA CCTTGGAAGT AGTAGATAAA GGATTCATTA ATATCTTCCC CATGATAAAC
961      ACTACAGTAT TTGTAAACGA TGGAGAAAAT GTAGATTTGA TTGTTGAATA TGAGGCATTC
1021     CCCAAACCTG AACACCAGCA GTGGATCTAT ATGAACAGAA CCGTCACTGA TAAATGGGAA
1081     GATTATCCCA AGTCTGAGAA TGAAAGTAAT ATCAGATACG TAAGTGAACT TCATCTAACC
1141     AGATTAAAGG GCACTGAAGG AGGCACTTAC ACATTCCTAG TGTCCAATTC TGACGTCAAT
1201     GCTTCCATAG CATTTAATGT TTATGTGAAT ACAAAACCAG AAATCCTGAC TTACGACAGG
1261     CTCGTGAATG GCATGCTCCA ATGTGTGGCA GCAGGATTCC CAGAACCCAC AATAGATTGG
1321     TATTTTTGTC CGGGAACTGA GCAGAGATGC TCTGCTTCTG TACTGCCAGT GGATGTGCAG
1381     ACACTAAACG CATCTGGGCC ACCGTTTGGA AAACTAGTGG TTCAGAGTTC CATAGATTCT
1441     AGTGCATTCA AGCACAATGG CACGGTTGAA TGTAAGGCTT ACAACGATGT GGGCAAGACT
1501     TCTGCCTATT TTAACTTTGC ATTTAAAGAG CAAATCCATC CCCACACCCT GTTCACTCCT
1561     TTGCTGATTG GTTTTGTAAT CGTAGCTGGC ATGATGTGCA TTATTGTGAT GATTCTCACC
1621     TACAAATATT TACAGAAACC CATGTATGAA GTACAGTGGA AGGTTGTTGA GGAGATAAAT
1681     GGAAACAATT ACGTTTACAT AGACCCAACA CAACTTCCTT ATGATCACAA ATGGGAGTTT
1741     CCCAGAAACA GGCTGAGTTT TGGGAAAACC CTGGGTGCTG GCGCTTTTGG GAAGGTTGTT
1801     GAGGCAACTG CTTATGGCTT AATTAAGTCG GACGCGGCCA TGACTGTCGC TGTAAAGATG
1861     CTCAAACCAA GTGCCCATTT AACAGAACGA GAAGCCCTCA TGTCTGAACT CAAAGTCCTG
1921     AGTTACCTTG GTAATCATAT GAATATTGTG AATCTTCTTG GAGCCTGCAC CATTGGAGGG
1981     CCCACCCTGG TCATTACAGA ATATTGTTGC TATGGTGATC TTTTGAATTT TTTGAGAAGA
2041     AAACGTGATT CTTTTATTTG TTCAAAGCAG GAAGATCATG CAGAATCTGC ACTTTATAAG
2101     AATCTTCTGC ATTCAAAGGA GTCTTCCTGC AGCGATAGTA CTAATGAGTA CATGGACATG
2161     AAACCTGGAG TTTCTTATGT CGTCCCAACC AAGGCTGACA AAAGGAGATC TGCGAGAATA
2221     GGCTCATATA TAGAAAGAGA TGTGACACCC GCCATCATGG AGGATGACGA GTTGGCTTTA
2281     GACTTAGAAG ACTTGCTGAG CTTTTCTTAC CAGGTGGCAA AGGGCATGGC TTTCCTTGCC
2341     TCCAAGAATT GTATTCACAG AGACTTGGCA GCCAGAAATA TCCTCCTTAC TCATGGTCGG
2401     ATCACAAAGA TTTGTGATTT TGGCCTAGCC AGAGACATCA AGAATGATTC TAATTATGTG
2461     GTTAAAGGAA ACGCTCGACT ACCTGTGAAG TGGATGGCAC CTGAAAGCAT TTTCAACTGT
2521     GTGTACACAT TTGAAAGTGA CGTCTGGTCC TATGGGATTT TTCTTTGGGA GCTATTCTCT
2581     TTAGGAAGCA GCCCCTATCC TGGAATGCCA GTCGATTCTA AATTCTACAA GATGATCAAG
2641     GAAGGCTTCC GGATGCTCAG CCCTGAACAT GCACCTGCTG AAATGTATGA CATAATGAAG
2701     ACTTGCTGGG ATGCGGATCC CCTAAAAAGA CCAACATTCA AGCAAATTGT TCAGCTAATT
2761     GAGAAGCAGA TTTCAGAGAG CACCAATCAT ATTTACTCCA ACTTAGCAAA CTGCAGCCCC
2821     AACCGACAGA AGCCTGTAGT GGACCATTCC GTGCGGATCA ATTCTGTCGG CAGCACCGCT
2881     TCCTCCTCCC AACCTCTGCT TGTGCACGAC GACGTCTGA

 

Quality Control:cDNA sequencing results is available upon request.