Rat KIT Gene cDNA Clone / ORF Clone

KIT cDNA Clone Datasheet

KIT cDNA Clone Product Information

• Gene Bank Ref.ID: NM_022264.1
• cDNA Size: 2937
• Gene Synonym: Kit
• Source: Rat

KIT cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutations: 2122 C/T resulting in the amino acid Leu substitution by Phe and 2190 C/G not causing the amino acid variation.

KIT cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KIT cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KIT cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAGGCG CTCGCGGCGC CTGGGATCTG CTCTGCGTCC TGTTGGTCCT GCTCCGTGGC
61       CAGACAGGGA CTTCTCAGCC ATCTGCGAGT CCAGGGGAGC CGTCTCCACC ATCCATCCAG
121      CCGGCCCAGT CAGAGTTAAT AGTTGAAGCC GGGGACACCA TCAGGCTGAC GTGCACTGAC
181      CCCGCCTTTG TCAAATGGAC TTTCGAGATC CTCGATGTAA GGATTGAGAA TAAGCAGAGC
241      GAATGGATTC GAGAAAAAGC CGAGGCCACT CACACGGGCA AATACACGTG CGTCAGCGGC
301      AGCGGCCTCA GGAGCTCTAT TTACGTGTTC GTTAGAGATC CTGCCGTACT TTTCCTGGTT
361      GGCCTTCCCT TGTTTGGCAA AGAAGACAAC GACGCACTGG TCCGCTGCCC CCTGACAGAC
421      CCACAGGTGT CCAATTACTC CCTCATTGAG TGTGATGGGA AATCTCTCCC CACGGACCTG
481      AAGTTCGTCC CCAACCCCAA GGCTGGCATC ACCATCAAAA ACGTGAAGCG CGCCTACCAC
541      CGGCTGTGCA TCCGGTGTGC TGCCCAGCGT GAGGGCAAAT GGATGCGGTC TGACAAATTC
601      ACCCTCAAAG TGAGAGCAGC CATCAAAGCT ATCCCAGTGG TGTCTGTGCC CGAAACAAGT
661      CATCTCCTTA AGGAAGGGGA CACATTTACG GTGATATGCA CCATAAAAGA CGTGTCTACA
721      TCCGTGGACT CCATGTGGAT AAAGTTGAAC CCTCAGCCTC AGAGCAAAGC CCAGGTAAAG
781      CGCAATAGCT GGCATCAGGG CGACTTCAAT TACGAACGCC AGGAGACGCT GACTATCAGC
841      TCAGCAAGAG TTAACGATTC CGGAGTGTTC ATGTGTTATG CCAATAATAC TTTTGGATCA
901      GCAAATGTCA CAACAACCTT GAAAGTAGTA GAAAAGGGAT TCATCAACAT CTTCCCTGTG
961      AAGAACACTA CGGTATTTGT AACTGATGGG GAAAATGTAG ACTTGGTTGT TGAGTTCGAG
1021     GCCTACCCTA AACCTGAACA CCAGCAGTGG ATCTACATGA ACAGGACGCC TACTAACAGA
1081     GGGGAGGATT ATGTCAAATC CGACAACCAA AGCAACATCA GATATGTGAA CGAACTTCGC
1141     CTGACCAGAT TGAAAGGCAC AGAAGGAGGC ACTTACACCT TTCTGGTGTC CAACTCTGAT
1201     GTCAGTGCTT CCGTGACATT TGATGTTTAT GTGAACACAA AACCAGAAAT CCTGACATAT
1261     GACAGGCTCA TGAATGGCAG GCTCCAGTGT GTGGCGGCGG GATTCCCGGA GCCCACAATA
1321     GATTGGTATT TTTGTACAGG GGCAGAGCAA AGGTGTACCG TTCCTGTCCC GCCAGTAGAC
1381     GTACAGATCC AGAATGCGTC TGTGTCACCA TTTGGAAAAC TGGTGGTTCA GAGTTCCATA
1441     GACTCCAGCG TCTTCCGGCA CAACGGCACG GTGGAGTGTA AGGCCTCCAA CGCTGTGGGC
1501     AAGAGCTCTG CCTTCTTTAA CTTTGCATTT AAAGGTAACA GCAAAGAGCA AATCCAGCCC
1561     CACACCCTGT TCACGCCGCT GCTCATTGGC TTCGTGGTCA CAGCCGGCTT GATGGGGATC
1621     ATTGTGATGG TTCTTGCCTA CAAATATTTG CAGAAACCCA TGTATGAAGT ACAATGGAAG
1681     GTTGTCGAGG AGATAAATGG GAACAATTAT GTTTACATAG ACCCAACGCA GCTTCCTTAT
1741     GACCACAAAT GGGAGTTTCC CAGAAACAGG CTGAGTTTTG GAAAGACCTT GGGAGCTGGT
1801     GCCTTTGGGA AGGTAGTTGA GGCCACTGCC TATGGCTTAA TTAAGTCGGA TGCCGCCATG
1861     ACGGTTGCCG TGAAGATGCT CAAACCAAGT GCCCATTTAA CGGAAAGGGA GGCCCTAATG
1921     TCAGAACTGA AGGTCCTGAG CTACCTGGGT AATCACATGA ATATCGTCAA CCTCCTTGGA
1981     GCGTGTACCG TGGGAGGGCC CACCCTGGTC ATTACAGAAT ACTGTTGCTA TGGTGATCTT
2041     TTGAATTTCT TGAGAAGAAA GCGTGACTCG TTTATTTTCT CAAAGCAAGA AGAACAGGCA
2101     GACGCCGCAC TTTATAAGAA CTTTCTGCAT TCAAAGGAGT CTTCCTGTGA CAGCTCAAAC
2161     GAGTACATGG ACATGAAGCC TGGCGTTTCG TACGTCGTAC CAACCAAGAC AGACAAAAGG
2221     AGATCCGCAA GAATAGACTC GTATATAGAA AGAGACGTGA CTCCCGCCAT CATGGAAGAT
2281     GACGAGCTGG CTCTGGACCT GGAAGATTTG CTGAGCTTTT CCTACCAGGT GGCCAAGGGC
2341     ATGGCGTTCC TCGCCTCCAA GAACTGTATT CACAGAGATT TGGCAGCCAG GAATATCCTC
2401     CTCACTCACG GGCGGATCAC AAAGATTTGC GATTTCGGCC TAGCCAGAGA CATCAGGAAT
2461     GATTCGAATT ACGTGGTAAA AGGAAATGCA CGGCTGCCCG TGAAGTGGAT GGCACCGGAG
2521     AGCATTTTCA ACTGCGTGTA CACATTTGAA AGTGACGTCT GGTCCTATGG GATTTTCCTC
2581     TGGGAGCTAT TCTCTCTAGG AAGCAGCCCC TACCCAGGGA TGCCGGTCGA TTCCAAGTTT
2641     TACAAGATGA TCAAGGAAGG TTTCCGAATG CTCAGCCCTG AGCACGCGCC TGCCGCAATG
2701     TATGAAGTTA TGAAGACTTG CTGGGATGCT GATCCCCTGA AAAGGCCAAC ATTCAAGCAG
2761     GTTGTTCAGC TCATTGAGAA GCAGATCTCA GACAGCAGCA AACATATTTA CTCCAACTTA
2821     GCAAACTGTA ACCCCAACCC AGAGAACCCC GTGGTGGTGG ACCATTCTGT GAGGGTCAAT
2881     TCCGTCGGCA GCAGCACCTC TTCCACACAG CCTCTCCTCG TGCATGAGGA CGCCTGA

 

Quality Control:cDNA sequencing results is available upon request.