Text Size:AAA

Rat KIT / c-KIT Protein (His Tag)

DatasheetSpecific ReferencesReviewsRelated ProductsProtocols
c-KitProtein Product Information
Synonym:KIT
Protein Construction:A DNA sequence encoding the rat KIT (Q63116) (Met1-Thr522) was expressed, fused with a polyhistidine tag at the C-terminus.
Species:Rat
Expressed Host:Human Cells
Form & Shipping:In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
c-KitProtein QC Testing
Purity:> 95 % as determined by SDS-PAGE
Endotoxin:< 1.0 EU per μg of the protein as determined by the LAL method
Stability:Samples are stable for up to twelve months from date of receipt at -70℃
Predicted N Terminal:Ser 25
Molecule Mass:The recombinant rat KIT comprises 509 amino acids and predicts a molecular mass of 56.9 kDa. The apparent molecular mass of the recombinant protein is approximately 83 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Formulation:Lyophilized from sterile PBS, pH 7.4
1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
2. Please contact us for any concerns or special requirements.
c-KitProtein Usage Guide
Storage:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
80321-R08H
Background

C-Kit is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). c-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains.and 1 protein kinase domain. It belongs to the protein kinase superfamily, tyr protein kinase family and CSF-1/PDGF receptor subfamily. C-Kit contains 5 Ig-like C2-type (immunoglobulin-like) domains and 1 protein kinase domain. C-Kit has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase. Antibodies to c-Kit are widely used in immunohistochemistry to help distinguish particular types of tumour in histological tissue sections. It is used primarily in the diagnosis of GISTs. In GISTs, c-Kit staining is typically cytoplasmic, with stronger accentuation along the cell membranes. C-Kit antibodies can also be used in the diagnosis of mast cell tumours and in distinguishing seminomas from embryonal carcinomas. Mutations in c-Kit gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Defects in KIT are a cause of acute myelogenous leukemia (AML). AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development. Note=Somatic mutations that lead to constitutive activation of KIT are detected in AML patients.

References
  • Andre C, et al. (1997) Sequence analysis of two genomic regions containing the KIT and the FMS receptor tyrosine kinase genes. Genomics. 39(2):216-26.
  • Yarden Y, et al. (1987) Human proto-oncogene c-kit: a new cell surface receptor tyrosine kinase for an unidentified ligand. EMBO J. 6(11):3341-51.
  • Leong KG, et al. (2008) Generation of a prostate from a single adult stem cell. Nature. 456(7223): 804-8.
  • Edling CE, et al. (2007) c-Kit--a hematopoietic cell essential receptor tyrosine kinase. Int J Biochem Cell Biol. 39(11):1995-8.
  • McIntyre A, et al. (2005) Amplification and overexpression of the KIT gene is associated with progression in the seminoma subtype of testicular germ cell tumors of adolescents and adults. Cancer Res. 65(18):8085-9.
  • Receptor Tyrosine Kinase (RTK) Related Products
    Product nameProduct name
    Human EphA1 / Eph Receptor A1 Protein (His Tag, ECD)Rat EphB3 / HEK2 / Eph Receptor B3 Protein (His Tag, ECD)Human VEGFR1 / FLT-1 Protein (Fc Tag)Human HER2 / ErbB2 ProteinCanine TrkB / NTRK2 Protein (Fc Tag)Rhesus HER3 / ErbB3 ProteinCynomolgus / Rhesus FGFR3 / CD333 Protein (His Tag)Human FGFR3 / CD333 Protein (alpha(IIIb), His Tag)Human FGFR2 / CD332 Protein (beta(IIIc), His Tag)Cynomolgus / Rhesus PDGFRB / CD140b Protein (Fc Tag)Human FGFR3 / CD333 Protein (alpha(IIIb), Fc Tag)Human HER3 / ErbB3 ProteinHuman FGFR2 / CD332 Protein (alpha(IIIb), Fc Tag)Cynomolgus / Rhesus HER2 / ErbB2 Protein (ECD, Fc Tag)Human EGFR / HER1 / ErbB1 (aa 668-1210) Protein (His & GST Tag)Mouse EphB6 Protein (His Tag)Cynomolgus / Rhesus c-MET / HGFR Protein (Fc Tag)Cynomolgus / Rhesus c-MET / HGFR Protein (His Tag)Human / Rhesus HER4 / ErbB4 Protein (His Tag)Cynomolgus / Rhesus c-MET / HGFR ProteinCynomolgus HER2 / ErbB2 Protein (His Tag)Human VEGFR1 / FLT-1 Protein (His Tag)Human c-MET / HGFR Protein (His & Fc Tag)Human EGFR / HER1 / ErbB1 Protein (His Tag)Human HER2 / ErbB2 Protein (Fc Tag)Human TrkB / NTRK2 Protein (His Tag)Human DDR2 Kinase / CD167b Protein (Fc Tag)Human EphB2 Protein (His & Fc Tag)Human EphB4 / HTK Protein (Fc Tag)Human EphB4 / HTK Protein (His Tag)Human EphB4 / HTK ProteinHuman EphB6 / EphB6 Protein (Fc Tag)Human EphB6 / EphB6 ProteinHuman HER3 / ErbB3 Protein (Fc Tag)Human HER4 / ErbB4 Protein (His & Fc Tag)Human FGFR1 / CD331 Protein (His & Fc Tag)Human FGFR4 / FGF Receptor 4 Protein (Fc Tag)Human FGFR4 / FGF Receptor 4 Protein (His & Fc Tag)Human IGF1R / CD221 Protein (His Tag)Human CSF1R / MCSF Receptor / CD115 Protein (His & Fc Tag)Human CSF1R / MCSF Receptor / CD115 ProteinHuman MERTK / Mer Protein (His & Fc Tag)Human PDGFRa / CD140a Protein (Fc Tag)Human PDGFRa / CD140a ProteinHuman Tie2 / CD202b Protein (His & Fc Tag)Human TrkC / NTRK3 Protein (His & Fc Tag)Human TrkC / NTRK3 Protein (His Tag)Human VEGFR3 / FLT4 Protein (His Tag)Human FGFR2 Protein (His & Fc Tag)Mouse FGFR3 / CD333 Protein (His & Fc Tag)Mouse FGFR4 / CD334 Protein (His Tag)Human FGFR1 / CD331 Protein (His Tag)Mouse FGFR4 / CD334 Protein (His & Fc Tag)Mouse Axl Kinase Protein (His & Fc Tag)Human CSF1R / MCSF Receptor / CD115 Protein (His Tag)Human EGFR / HER1 / ErbB1 Protein (Fc Tag)Human VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)Human Tie2 / CD202b / TEK Protein (His Tag)Human EphB2 Protein (His Tag)Human VEGFR3 / FLT4 Protein (Fc Tag)Human TrkA / NTRK1 Protein (His & Fc Tag)Human TrkA / NTRK1 Protein (His Tag)Human TrkB / NTRK2 Protein (His & Fc Tag)Human HER2 / ErbB2 Protein (His Tag)Human PDGFRa / CD140a Protein (His Tag)Human Insulin Receptor / INSR / CD220 Protein (long isoform, His Tag)Human Insulin Receptor / INSR / CD220 Protein (short isoform, His Tag)Human CD140b / PDGFRB Protein (His Tag)Mouse FGFR3 / CD333 Protein (His Tag)Mouse TrkC / NTRK3 Protein (His Tag)Human FGFR2 / CD332 Protein (His Tag)Human DDR1 Kinase / MCK10 / CD167 Protein (His Tag)Human c-MET / HGFR Protein (His Tag)Mouse EphA6 / EHK-2 Protein (His Tag)Mouse FGFRL1 / FGFR5 Protein (His Tag)Mouse Axl Kinase Protein (His Tag)Human HER3 / ErbB3 Protein (His Tag)Human EphB6 / EphB6 Protein (His Tag)Human EphA4 Protein (His Tag)Human EGFR / HER1 / ErbB1 Protein (His Tag)Human Axl Kinase Protein (His Tag)Human EphA7 / EHK3 Protein (His Tag)Mouse DDR2 Kinase / CD167b Protein (His Tag)Human TrkA / NTRK1 Protein (aa 285-413, His Tag)Human TrkA / NTRK1 Protein (aa 194-413, His Tag)Mouse EphA2 Protein (His Tag)Mouse EphA4 / HEK8 Protein (His Tag)Mouse EphA4 / HEK8 Protein (Fc Tag)Mouse FGFR1 / CD331 Protein (His Tag)Mouse FGFR1 / CD331 Protein (Fc Tag)Mouse EphB3 / HEK2 Protein (His Tag)Mouse EphB4 / HTK Protein (Fc Tag)Mouse EphB4 / HTK Protein (His Tag)Mouse EphA6 / EHK-2 Protein (Fc Tag)Mouse EphA7 / EHK-3 Protein (His Tag)Mouse c-kit / CD117 Protein (Fc Tag)Mouse c-kit / CD117 Protein (His Tag)Mouse CSF1R / MCSF Receptor / CD115 Protein (His Tag)Human MUSK Kinase Protein (aa 433-783, His & GST Tag)Mouse c-MET / HGFR Protein (Fc Tag)Mouse c-MET / HGFR Protein (His Tag)Human Tie1 Protein (His Tag)Human c-KIT / CD117 Protein (His Tag)Mouse VEGFR3 / FLT-4 Protein (His Tag)Mouse VEGFR3 / FLT-4 Protein (Fc Tag)Rat HER2 / ErbB2 ProteinRat HER2 / ErbB2 Protein (Fc Tag)Rat HER2 / ErbB2 Protein (His Tag)Rhesus HER2 / ErbB2 Protein (Fc Tag)Rhesus HER2 / ErbB2 Protein (His Tag)Mouse Tie2 / CD202b / TEK Protein (ECD, Fc Tag)Human EphB1 / EPHT2 Protein (His Tag)Human PDGFRB / CD140b Protein (His & Fc Tag)Human CD136 / MST1R Protein (His Tag)Human EphA4 Protein (His & Fc Tag)Canine c-MET / HGFR Protein (His Tag)Human VEGFR1 / FLT-1 Protein (His Tag)Human FGFR4 / FGF Receptor 4 Protein (His Tag)Mouse EphB1 / EPHT2 Protein (His & GST Tag)Human RET Kinase Protein (aa 658-1114, His & GST Tag)Mouse EphB1 / EPHT2 Protein (His Tag)Mouse TrkB / NTRK2 Protein (His Tag)Human RET Kinase Protein (His Tag)Mouse MERTK / Mer Protein (Fc Tag)Mouse DDR1 Kinase / MCK10 / CD167 Protein (His Tag)Mouse HER2 / ErbB2 / CD340 Protein (His Tag)Human VEGFR2 / Flk-1 / CD309 / KDR Protein (His Tag)Mouse HER3 / ErbB3 Protein (His Tag)Mouse HER2 / ErbB2 Protein (Fc Tag)Rhesus HER3 / ErbB3 Protein (His Tag)Rhesus HER3 / ErbB3 Protein (Fc Tag)Mouse DDR1 Kinase / MCK10 / CD167 Protein (Fc Tag)Rat Tie2 / TEK Protein (Fc Tag)Mouse EphA1 / EPH receptor A1 Protein (His Tag)Human MERTK / Mer Protein GST TagRat FGFR4 / FGF Receptor 4 Protein (Fc Tag)Human CSF1R / MCSF Receptor / CD115 Protein (His & GST Tag)Human FGFR1 / CD331 Protein (His & GST Tag)Human Insulin Receptor / INSR / CD220 Protein (His & GST Tag)Mouse EGFR / HER1 / ErbB1 Protein (Fc Tag)Mouse EGFR / HER1 / ErbB1 Protein (His Tag)Rat VEGFR2 / Flk-1 / CD309 / KDR Protein (His Tag)Human HER2 / ErbB2 / CD340 (676-1255) Protein (His & GST Tag)Human KIT / c-KIT / CD117 Protein (aa 540-972, His & GST Tag)Mouse VEGFR2 / Flk-1 / CD309 / KDR Protein (His Tag)Human CSF1R / MCSF Receptor / CD115 Protein (aa 543-922, His & GST Tag)Human / Rhesus HER4 / ErbB4 Protein (His Tag)Human / Rhesus HER4 / ErbB4 Protein (Fc Tag)Human HER3 / ErbB3 Protein (aa 730-1065, His & GST Tag)Human FGFR2 / CD332 Protein (aa 400-821, His & GST Tag)Human VEGFR2 / Flk-1 / CD309 / KDR Protein (His & GST Tag)Cynomolgus FGFR3 Protein (Fc Tag)Human Tie2 / CD202b / TEK Protein (His & GST Tag)Human EphA7 / EHK3 Protein (His & GST Tag)Mouse MERTK / Mer Protein (His & GST Tag)Mouse VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)Rat FGFR4 / FGF Receptor 4 Protein (His Tag)Mouse Tie2 / TEK Protein (His Tag)Mouse Tie2 / TEK Protein (aa 770-1122, His & GST Tag)Mouse DDR1 Kinase / MCK10 / CD167 Protein (His & GST Tag)Rat TrkB / NTRK2 Protein (Fc Tag)Human DDR2 Kinase / CD167b Protein (aa 422-855, His & GST Tag)Human c-MET / HGFR Protein (aa 956-1390, His & GST Tag)Human DDR1 Kinase / MCK10 / CD167 Protein (aa 444-913, His & GST Tag)Human EphA4 / HEK8 Protein (aa 570-986, His & GST Tag)Rat EphA4 Protein (His Tag)Cynomolgus EphA4 Protein (Fc Tag)Rat EGFR / HER1 / ErbB1 Protein (His Tag)Human PDGFRa / CD140a Protein (His & GST Tag)Rat EphA4 Protein (Fc Tag) Human EphB4 / HTK Protein (aa 563-987, His & GST Tag)Human EphB2 / Hek5 Protein (aa 570-987, His & GST Tag)Mouse HER4 / ErbB4 Protein (His Tag)Rat HER3 / ErbB3 Protein (Fc Tag)Rat HER3 / ErbB3 Protein (His Tag)Rat KIT / c-KIT Protein (Fc Tag)Human EphB1 / EPHT2 Protein (aa 565-984, His & GST Tag)Human EphB3 / HEK2 Protein (aa 585-998, His & GST Tag)Human EphA2 Protein (aa 585-976, His & GST Tag)Rat DDR2 Kinase / CD167b Protein (Fc Tag)Rat KIT / c-KIT Protein (His Tag)Rat PDGFRB / PDGFR-1 Protein (His Tag)Cynomolgus EphB6 / EphB6 Protein (Fc Tag)Rat HER4 / ErbB4 Protein (Fc Tag)Rat TrkB / NTRK2 Protein (His Tag)Rat DDR2 Kinase / CD167b Protein (His Tag)Mouse CSF1R / MCSF Receptor / CD115 Protein (His & Fc Tag)Rat VEGFR2 / Flk-1 / CD309 / KDR Protein (Fc Tag)Rat VEGFR1 / FLT-1 Protein (His Tag)Rat DDR1 Kinase / MCK10 / CD167 Protein (His Tag)Cynomolgus EphA4 Protein (His Tag)Cynomolgus FGFR4 / FGF Receptor 4 Protein (Fc Tag)Cynomolgus FGFR1 / CD331 Protein (Fc Tag)Mouse FGFR2 / CD332 Protein (Fc Tag)Mouse FGFR2 / CD332 Protein (His Tag)Rat TrkA / NTRK1 Protein (His Tag)Mouse TrkA / NTRK1 Protein (Fc Tag)Rat PDGFRB / PDGFR-1 Protein (Fc Tag)Human MERTK / Mer(aa 578-872) ProteinHuman PDGFRB / PDGFR-1 / CD140b ProteinHuman EphB2 / Hek5 ProteinRat EphA7 / EHK3 Protein (His Tag)Mouse TrkA / NTRK1 Protein (His Tag)Rat DDR1 Kinase / MCK10 / CD167 Protein (Fc Tag) Human EphA2 Protein (His Tag)Rat TrkA / NTRK1 Protein (Fc Tag)Cynomolgus Tie2 / TEK Protein (Fc Tag)Human KIT / c-KIT / CD117 Protein (aa 50-190, His Tag)Rat c-MET / HGFR Protein (Fc Tag)Cynomolgus PDGFRB / PDGFR-1 Protein (His Tag)Cynomolgus DDR2 Kinase / CD167b Protein (His Tag)Mouse EphB2 / Hek5 Protein (Fc Tag)Human DDR2 Kinase / CD167b Protein (His Tag)Canine TrkB / NTRK2 Protein (His Tag)Cynomolgus DDR2 Kinase / CD167b Protein (Fc Tag)Cynomolgus FGFR4 / FGF Receptor 4 Protein (His Tag)Canine TrkA / NTRK1 Protein (Fc Tag)Canine TrkA / NTRK1 Protein (His Tag)Canine HER2 / ErbB2 Protein (His Tag)Canine EGFR / HER1 / ErbB1 Protein (His Tag)Rat CSF1R / MCSF Receptor / CD115 Protein (His Tag)Human HER3 / ErbB3 Protein (Fc Tag)Rat HER4 / ErbB4 Protein (His Tag)Rat PDGFRa / CD140a Protein (His Tag)Cynomolgus EphB1 / EPHT2 Protein (Fc Tag)Rat PDGFRa / CD140a Protein (Fc Tag)Rat CSF1R / MCSF Receptor / CD115 Protein (Fc Tag)Cynomolgus EphB1 / EPHT2 Protein (His Tag)Cynomolgus FGFR1 / CD331 Protein (His Tag)Human KIT / c-KIT / CD117 Protein (Fc Tag)Human FGFR3 / CD333 Protein (His Tag, ECD)Human FGFR3 / CD333 Protein (Fc Tag, ECD)Mouse EphB2 / Hek5 Protein (His Tag)
    Catalog:80321-R08H-20
    List Price: $108.00  (Save $0.00)
    Price:$108.00      [How to order]
    Availabilityon Sale
    Images
    • Rat KIT / c-KIT Protein (His Tag) SDS-PAGE
    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"