Rat KIT / c-KIT Protein (His Tag)

V-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog Protein Datasheet

KIT / c-KIT Protein Product Information

• Synonym: KIT
• Protein Construction: A DNA sequence encoding the rat KIT (Q63116)(Met1-Thr522) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

KIT / c-KIT Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Ser 25
• Molecular Mass: The recombinant rat KIT comprises 509 amino acids and predicts a molecular mass of 56.9 kDa. The apparent molecular mass of the recombinant protein is approximately 83 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KIT / c-KIT protein

KIT / c-KIT Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KIT / c-KIT Protein Description

Mast / stem cell growth factor receptor, also known as Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, CD117 and KIT, is a single-pass type I membrane protein which belongs to the protein kinase superfamily, Tyr protein kinase family and CSF-1/PDGF receptor subfamily. CD117 / KIT contains five Ig-like C2-type (immunoglobulin-like) domains and one protein kinase domain. CD117 / KIT is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K). CD117 is the receptor for the cytokine stem cell factor (SCF), when this receptor binds to SCF it forms a dimer that activates signaling through second messengers. Signaling through CD117 plays a role in cell survival, proliferation, and differentiation. Defects in CD117 / KIT are a cause of piebaldism which is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. Defects in CD117 / KIT have also been associated with testicular tumors (TEST) which is a common solid malignancy in males.

References

1. Fleischman RA. et al., 1992, J Clin Invest. 89:1713-7.
2. Spritz RA. et al., 1992, Am J Hum Genet. 50:261-9.
3. Syrris P. et al., 2000, Am J Med Genet. 95:79-81.
4. Leong KG. et al., 2008, Nature. 456 (7223): 804.