Rat KIRREL3 Gene cDNA Clone / ORF Clone

KIRREL3 cDNA Clone Datasheet

KIRREL3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001048215.1
• cDNA Size: 2301
• Gene Synonym: Kirrel3
• Source: Rat

KIRREL3 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus kin of IRRE like 3 (Drosophila) DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 2133 A/G not causing the amino acid variation.

KIRREL3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KIRREL3 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KIRREL3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGACCTT TCCAGCTGGA TTTGCTCTTC CTCTGCTTCT TCCTTTTCAG TCAAGAGCTT
61       GGCCTCCAGA GGAGAGGATG CTGTCTGGTG CTGGGCTACA TGGCCAAGGA CAAGTTTCGG
121      AGAATGAATG AAGGTCAAGT CTACTCCTTC AGCCAGCAAC CCCAGGACCA AGTGGTGGTG
181      TCAGGACAAC CAGTGACTCT GCTGTGTGCC ATCCCTGAAT ATGATGGCTT CGTCCTGTGG
241      ATCAAAGATG GCTTGGCTCT AGGTGTAGGC AGAGACCTCT CAAGCTACCC CCAGTACCTG
301      GTGGTGGGGA ACCACCTCTC AGGAGAGCAT CACCTGAAGA TCCTGAGGGC TGAGCTGCAG
361      GACGATGCTG TGTACGAGTG CCAGGCCATC CAGGCTGCCA TCCGGTCCCG CCCTGCACGC
421      CTTACAGTCC TGGTGCCACC AGATGACCCC ATCATCCTAG GGGGGCCTGT GATCAGCCTT
481      CGGGCAGGGG ATCCCCTCAA CCTCACCTGC CATGCAGACA ATGCCAAGCC TGCAGCTTCC
541      ATCATCTGGC TACGCAAAGG AGAGGTCATC AATGGGGCCA CCTACTCCAA GACCCTGCTT
601      CGAGATGGCA AGCGTGAGAG CATCGTCAGC ACACTCTTCA TCTCCCCAGG GGACGTAGAA
661      AATGGGCAGA GCATTGTGTG CCGAGCCACC AACAAAGCCA TCCCCGGAGG AAAGGAGACC
721      TCAGTCACCA TAGACATCCA GCATCCACCA CTTGTCAACT TGTCCGTAGA ACCACAGCCG
781      GTATTGGAGG ACAATATCGT CACGTTCCAC TGCTCTGCAA AGGCCAACCC AGCTGTCACC
841      CAGTACAGGT GGGCCAAACG GGGTCACATC ATCAAGGAGG CATCTGGGGA GCTGTATAGG
901      ACCACCGTGG ACTACACATA CTTCTCAGAG CCTGTATCCT GCGAAGTGAC CAATGCCTTG
961      GGCAGCACCA ACCTCAGCCG CACAGTGGAC GTGTACTTTG GCCCTCGAAT GACCTCGGAG
1021     CCTCAGTCCC TGCTGGTAGA TCTGGGCTCA GATGCTGTCT TCAGCTGTGC GTGGATCGGC
1081     AACCCATCTC TGACCATCGT GTGGATGAAA CGAGGTTCTG GTGTGGTCCT GAGCAATGAA
1141     AAGACCCTAA CCCTCAAATC TGTCCGCCAA GAGGATGCCG GGAAGTACGT GTGCCGGGCT
1201     GTGGTGCCCC GGGTAGGAGC TGGGGAGAGA GAGGTGACCT TGACTGTCAA TGGACCCCCC
1261     ATCATCTCCA GCACACAGAC CCAGCATGCC CTCCATGGAG AGAAGGGCCA GATCAAATGC
1321     TTCATCCGGA GCACACCACC GCCTGACCGA ATTGCCTGGT CCTGGAAGGA GAATGTGCTG
1381     GAGTCGGGGA CATCAGGGCG CTACACAGTG GAGACGGTGA ACACCGAGGA GGGCGTCATC
1441     TCCACGCTGA CCATTAGCAA CATTGTGCGT GCTGACTTCC AGACCATATA CAACTGTACA
1501     GCCTGGAACA GCTTCGGCTC TGACACAGAG ATCATCCGAC TCAAGGAACA AGAGTCAGTG
1561     CCAATGGCCG TCATCATCGG GGTGGCCGTA GGAGCTGGCG TGGCCTTCCT CGTCCTAATG
1621     GCAACCATTG TGGCCTTCTG CTGTGCCCGT TCCCAGAGAA ATCTCAAAGG TGTTGTGTCA
1681     GCCAAAAATG ATATTCGAGT GGAGATTGTA CACAAGGAGC CAGCTTCTGG TCGGGAGGCT
1741     GAGGACCACA CCACCATCAA GCAGCTGATG ATGGACCGGG GTGAATTCCA ACAAGACTCC
1801     GTGCTGAAGC AGCTGGAGGT CCTCAAAGAA GAGGAGAAGG AGTTTCAGAA CCTGAAGGAC
1861     CCCACCAACG GCTACTACAG CGTCAACACC TTCAAAGAAC ACCATTCAAC TCCAACCATC
1921     TCCCTGTCCA GCTGCCAGCC AGACCTGCGT CCCACAGGCA AACAGCGAGT GCCCACAGGC
1981     ATGTCCTTCA CCAACATCTA CAGCACCTTG AGCGGCCAGG GCCGCCTCTA CGACTATGGA
2041     CAGAGGTTTG TACTGGGCAT GGGCAGCTCT TCCATTGAGC TTTGTGAGCG GGAGTTTCAG
2101     AGGGGCTCCC TCAGCGACAG CAGCTCCTTC CTGGACACGC AGTGTGACAG CAGCGTCAGC
2161     AGCAGCGGCA AACAAGATGG CTACGTGCAG TTTGACAAGG CCAGCAAGGC TTCTGCCTCC
2221     TCTTCCCACC ACTCCCAGTC CTCTTCCCAG AACTCCGACC CCAGTCGACC CCTGCAGCGG
2281     CGGATGCAGA CTCACGTCTG A

 

Quality Control:cDNA sequencing results is available upon request.