Rat KIRREL3 / NEPH2 Protein (His Tag)

Kin of IRRE like 3 (Drosophila) Protein Datasheet

KIRREL3 / NEPH2 Protein Product Information

• Synonym: KIRREL3
• Protein Construction: A DNA sequence encoding the rat KIRREL3(Q09GS6)(Met1-Ala523) was expressed with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

KIRREL3 / NEPH2 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 22
• Molecular Mass: The recombinant rat KIRREL3 comprises 513 amino acids and predicts a molecular mass of 56.4 kDa. The apparent molecular mass of the recombinant protein is approximately 60-64 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH 7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KIRREL3 / NEPH2 protein

KIRREL3 / NEPH2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KIRREL3 / NEPH2 Protein Description

Kin of IRRE-like protein 3, also known as Kin of irregular chiasm-like protein 3, Nephrin-like protein 2 and KIRREL3, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. KIRREL3 contains five Ig-like C2-type ( immunoglobulin-like ) domains. KIRREL3 is expressed in fetal and adult brain. It is also expressed in kidney, specifically in podocytes of kidney glomeruli. KIRREL3 could be involved in the hematopoetic supportive capacity of stroma cells. KIRREL3 might play a role in the axonal pathfinding, cell recognition, and synapse formation of DRG neurons on appropriate target cells, including the targeting of proprioceptive neurons on muscle spindles through the interaction with nephrin. Defects in KIRREL3 are the cause of mental retardation autosomal dominant type 4 (MRD4) which is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental perio.

References

1. Sellin L. et al., 2003, FASEB J. 17: 115-117.
2. Komori T. et al., 2008, J Comp Neurol. 511 (1): 92-108.
3. Bhalla K. et al., 2008, Am. J. Hum. Genet. 83: 703-713.