KIRREL1 / NEPH1 Protein

Kin of IRRE Like 1 / Nephrin-like protein 1

KIRREL1 / NEPH1 Products

KIRREL1 / NEPH1 Protein, Recombinant

Molecule	Species	Description	Cat. No
KIRREL1/NEPH1	Mosue	KIRREL1/NEPH1 Protein, Recombinant	50193-M08H

  50193-M08H:  When cells are added to KIRREL1 coated plates (30 µg/mL, 100 µL/well), 60%-70% will adhere specifically after 90 minutes at 37 ℃. Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells (ATCC: CRL2279).

KIRREL1 / NEPH1 cDNA Clone

Molecule	Species	Description	Cat. No
KIRREL1/NEPH1	Mosue	Mus musculus KIRREL1/NEPH1 cDNA Clone	MG50193-M

KIRREL1 / NEPH1 Alternative Names

KIRREL1, KIRREL, NEPH1, RP11-444M10.2, FLJ10845, MGC129542, MGC129543 [Homo sapiens]

Kirrel1, Kirrel, Neph1, 6720469N11Rik [Mus musculus]

KIRREL / Neph1 Related Areas

Immunology>>Adhesion Molecule>>Cell Adhesion Molecule (IgSF CAM)>>KIRREL1 / NEPH1

KIRREL1 / NEPH1 Background

NEPH1 (KIRREL1) belongs to a family of three closely related transmembrane proteins of the Ig superfamily with a structure similar to that of nephrin. All three Neph proteins share a conserved podocin-binding motif; mutation of a centrally located tyrosine residue dramatically lowers the affinity of Neph1 for podocin. Neph1 triggers AP-1 activation similarly to nephrin but requires the presence of Tec family kinases for efficient transactivation. Neph1 consists of a signal peptide, five Ig-like C2-type domains with the middle domain overlapping with a PKD-like domain, an RGD sequence, a transmembrane domain and a cytoplasmic tail, which is expressed in slit diaphragm domains of podocytes and in vertebrate and invertebrate nervous systems. Neph1 is abundantly expressed in the kidney, specifically expressed in podocytes of kidney glomeruli, and plays a significant role in the normal development and function of the glomerular permeability. Neph1 interacts with nephrin in vitro and in vivo, and able to stimulate transcriptional activation in a model system, such as the activation the transcription factor AP-1 via the stimulation of a MAPK module. Neph1 is crucial for the integrity of the slit diaphragm, as Neph1 gene knockout mice results in effacement of glomerular podocytes, heavy proteinuria, and early postnatal death.

KIRREL1 / NEPH1 Related Studies

1. Sellin L, et al. (2003) NEPH1 defines a novel family of podocin interacting proteins. FASEB J. 17(1): 115-7.
2. Kim EY, et al. (2009) Neph1 regulates steady-state surface expression of Slo1 Ca(2+)-activated K(+) channels: different effects in embryonic neurons and podocytes. Am J Physiol Cell Physiol. 297(6): C1379-88.