KIR2DL3 Antibody (Antigen Affinity Purified)

KIR2DL3 Antibody ( Antigen Affinity Purified ) Datasheet

KIR2DL3 Antibody Product Information

• Immunogen: Recombinant Human KIR2DL3 protein (Catalog#12828-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human KIR2DL3 (rh KIR2DL3; Catalog#12828-H08H; AAX23102.1; Met 1-His 245). KIR2DL3 specific IgG was purified by Human KIR2DL3 affinity chromatography.

KIR2DL3 Antibody Usage Guide

• Specificity: Human KIR2DL3
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KIR2DL3 in WB. Using a DAB detection system, the detection limit for Human KIR2DL3 is approximately 1 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human KIR2DL3. The detection limit for Human KIR2DL3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KIR2DL3 Antibody Background

Killer cell immunoglobulin-like receptor 2DL3, also known as CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, MHC class I NK cell receptor, NKAT2a, NKAT2b, Natural killer-associated transcript 2, p58 natural killer cell receptor clone CL-6, p58.2 MHC class-I-specific NK receptor, CD158b2 and KIR2DL3, is a single-pass type I membrane protein which belongs to theimmunoglobulin superfamily. KIR2DL3 contains 2Ig-like C2-type (immunoglobulin-like) domains. KIR2DL3 interacts with ARRB2. KIR2DL3 is a receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). KIR2DL3 inhibits the activity of NK cells thus preventing cell lysis.

References

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2. Wilson M.J., et al., 1997, Tissue Antigens 49: 574-579.
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4. Vitale,M. et al.,2004, Int Immunol. 16 (10): 1459-66.
5. Yu M.-C., et al., 2008, Nat. Immunol. 9: 898-907.