KIR2DL1 / CD158a Protein

Kller cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 1 Protein Datasheet

KIR2DL1 / CD158a Protein Price Inquiry ( Available Sizes )

• 200μg:  Inquiring Price;
• ≥1mg Bulk:  Inquiring Price

KIR2DL1 / CD158a Protein Product Information

• Synonym: XXbac-BPG184J6.7, CD158A, KIR-K64, KIR221, NKAT, NKAT1, p58.1
• Protein Construction: A DNA sequence encoding the human KIR2DL1 (NP_055033.2) extracellular domain (Met 1- His 245) was fused with the Fc region of human IgG1 at the C-terminus.
• Source: Human
• Expression Host: Human Cells

KIR2DL1 / CD158a Protein QC Testing

• Purity: >90 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: His 22
• Molecular Mass: The secreted recombinant human KIR2DL1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 465 amino acids and has a predicted molecular mass of 51.7 kDa. ?The apparent molecular mass of rhKIR2DL1/Fc monomer is approximately 70 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: KIR2DL1 / CD158a protein

KIR2DL1 / CD158a Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

KIR2DL1 / CD158a Protein Description

Inhibitory natural Killer cell Immunoglobulin-like Receptor with two Ig domains, KIR2DL1 is a transmembrane glycoprotein expressed by natural killer cells and subsets of T cells. Inhibitory NK cell receptors(KIR) down-regulate the cytotoxicity of NK cells upon recognition of specific class I major histocompatibility complex (MHC) molecules on target cells. It has been reported that the inhibitory NK cell immunoglobulin-like receptor 2DL1 (KIR2DL1) bound to its class I MHC ligand, HLA-Cw4. The KIR2DL1–HLA-Cw4 interface exhibits charge and shape complementarity. Specificity is mediated by a pocket in KIR2DL1 that hosts the Lys80 residue of HLA-Cw4. Many residues conserved in HLA-C and in KIR2DL receptors make different interactions in KIR2DL1–HLA-Cw4 and in a previously reported KIR2DL2–HLA-Cw3 complex. A dimeric aggregate of KIR–HLA-C complexes was observed in one KIR2DL1–HLA-Cw4 crystal.

References

1. Fan QR. et al., 2001, Nature Immunology. 2: 452-60.
2. Wagtmann N. et al., 1995, Immunity. 2 (5): 439-49.
3. Colonna M. et al., 1995, Science. 268 (5209): 405-8.