Mouse KIM-1 / TIM1 / HAVCR1 Protein

Hepatitis A Virus Cellular Receptor 1 ( TIM1 / HAVCR1 ) Protein Datasheet

TIM1 / HAVCR1 Protein Product Information

• Synonym: Havcr1 , RP23-31K14.2 , AI503787 , TIM-1 , Tim1 , Timd1
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse HAVCR1 isoform b (NP_001160104.1) (Tyr 22-Thr 212) was fused with a polyhistidine tag at the C-terminus and the Fc region of human IgG1 at the N-terminus
• Source: Mouse
• Expression Host: Human Cells

TIM1 / HAVCR1 Protein QC Testing

• Purity: > 97 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per 1μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Glu 20
• Molecular Mass: The recombinant mouse HAVCR1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 438 amino acids and predicts a molecular mass of 49 kDa. As a result of glycosylation, the apparent molecular mass of HAVCR1/Fc monomer is approximately 80-85 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile 20mM Tris-Citrate, 150mM NaCl, pH 6.5
• SDS-PAGE:

TIM1 / HAVCR1 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

TIM1 / HAVCR1 Protein Description

Mouse hepatitis A virus cellular receptor 1 (HAVCR1), also known as T cell immunoglobulin and mucin domain-containing protein 1 (TIMD1) and T cell membrane protein 1(TIM1), is a member of the TIM family , an immunologically important group of receptors that is conserved in vertebrates. TIM1 is widely expressed with highest levels in kidney and testis. It has been shown that mouse HAVCR1 is expressed by stimulated T-cells during primary antigen stimulation and associates with immunomodulatory properties. TIM1 is a type 1 integral membrane glycoprotein, and in the ECD contains one V-type I g-like domain extended above the cell surface by a mucin-like domain characterized by a variable number of threonine, serine, and proline (TSP) hexameric repeats. Extensive O-linked glycosylation resulted in the 70-80 kDa molecular mass more higher than the calculated MW(33KDa). TIM1 plays a critical role in T-helper cell development and the regulation of asthma and allergic diseases. In addition to HAV, another identified ligand for TIM1 is TIMD4. Mouse TIM1 ECD shares 50% and 80% amino acid sequence homology with human and rat TIM1 ECD rexpectively.

References

1. Silberstein, E. et al., 2001, J Virol.  75 (2): 717-725.
2. Santiago, C. et al., 2007, Immunity. 26:299-310.
3. Tami, C. et al., 2007, J Virol. 81 (7): 3437-3446.
4. Meyers, J.H. et al., 2005, Nat. Immunol. 6:455-464.