KIM-1 / TIM1 / HACVR1 Antibody (Antigen Affinity Purified)

KIM-1 / TIM1 / HACVR1 Antibody (Antigen Affinity Purified) Datasheet

KIM-1 / TIM1 / HACVR1 Antibody Product Information

• Immunogen: Recombinant Mouse KIM-1 / TIM1 / HACVR1 protein (Catalog#50321-M07H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Mouse KIM-1 / TIM1 / HACVR1 (rM KIM-1 / TIM1 / HACVR1; Catalog#50321-M07H; NP_001160104.1; Tyr 22-Thr 212). KIM-1 / TIM1 / HACVR1 specific IgG was purified by Mouse KIM-1 / TIM1 / HACVR1 affinity chromatography.

KIM-1 / TIM1 / HACVR1 Antibody Usage Guide

• Specificity: Mouse KIM-1 / TIM1 / HACVR1
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse KIM1 in WB. Using a DAB detection system, the detection limit for Mouse KIM1 is approximately 0.5 ng/lane under non-reducing conditions and reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse KIM1. The detection limit for Mouse KIM1 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KIM-1 / TIM1 / HACVR1 Antibody Background

Hepatitis A virus cellular receptor 1 (HAVCR1), also known as T cell immunoglobulin and mucin domain-containing protein 1 (TIMD1) and T cell membrane protein 1 (TIM1), is a member of the TIM family, an immunologically important group of receptors that is conserved in vertebrates. TIM1 is widely expressed with highest levels in kidney and testis. It has been shown that mouse HAVCR1 is expressed by stimulated T-cells during primary antigen stimulation and associates with immunomodulatory properties. TIM1 is a type 1 integral membrane glycoprotein, and in the ECD contains one V-type Ig-like domain extended above the cell surface by a mucin-like domain characterized by a variable number of threonine, serine, and proline (TSP) hexameric repeats. TIM1 plays a critical role in T-helper cell development and the regulation of asthma and allergic diseases. In addition to HAV, another identified ligand for TIM1 is TIMD4. Rat TIM1 ECD shares 50% and 81% amino acid sequence homology with human and mouse TIM1 ECD rexpectively.

References

1. Silberstein, E. et al., 2001, J Virol.75 (2): 717-725.
2. Silberstein, E. et al., 2003, J Virol.77 (16): 8765-8774.
3. Tami, C. et al., 2007, J Virol.81 (7): 3437-3446.
4. Garner-Spitzer, E. et al., 2009, Vaccine.27 (2): 197-204.