KIM-1 / TIM1 / HACVR1 Antibody

KIM-1 / TIM1 / HACVR1 Antibody Datasheet

KIM-1 / TIM1 / HACVR1 Antibody Product Information

• Immunogen: Recombinant Human KIM-1 / TIM1 / HACVR1 protein (Catalog#11051-H07H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 10B8C11
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human KIM-1 / TIM1 / HACVR1 (rh KIM-1 / TIM1 / HACVR1; Catalog#11051-H07H; AAC39862.1; Ser 21-Gly 290). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

KIM-1 / TIM1 / HACVR1 Antibody Usage Guide

• Specificity: Human KIM-1 / TIM1 / HACVR1
• No cross-reactivity in ELISA with

  Mouse KIM1

  Human TIMD3

  Rat KIM1

  Human TIMD4

  Human cell lysate (293 cell line)
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human KIM1. The detection limit for Human KIM1 is approximately 0.078 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KIM-1 / TIM1 / HACVR1 Antibody Background

Hepatitis A virus cellular receptor 1 (HAVCR1), also known as T cell immunoglobulin and mucin domain-containing protein 1 (TIMD1) and T cell membrane protein 1 (TIM1), is a member of the TIM family, an immunologically important group of receptors that is conserved in vertebrates. TIM1 is widely expressed with highest levels in kidney and testis. It has been shown that mouse HAVCR1 is expressed by stimulated T-cells during primary antigen stimulation and associates with immunomodulatory properties. TIM1 is a type 1 integral membrane glycoprotein, and in the ECD contains one V-type Ig-like domain extended above the cell surface by a mucin-like domain characterized by a variable number of threonine, serine, and proline (TSP) hexameric repeats. TIM1 plays a critical role in T-helper cell development and the regulation of asthma and allergic diseases. In addition to HAV, another identified ligand for TIM1 is TIMD4. Rat TIM1 ECD shares 50% and 81% amino acid sequence homology with human and mouse TIM1 ECD rexpectively.

References

1. Silberstein, E. et al., 2001, J Virol.75 (2): 717-725.
2. Silberstein, E. et al., 2003, J Virol.77 (16): 8765-8774.
3. Tami, C. et al., 2007, J Virol.81 (7): 3437-3446.
4. Garner-Spitzer, E. et al., 2009, Vaccine.27 (2): 197-204.