KIM-1 / TIM1 / HACVR1 Antibody

KIM-1 / TIM1 / HACVR1 Antibody Datasheet

KIM-1 / TIM1 / HACVR1 Antibody Product Information

• Immunogen: Recombinant Cynomolgus KIM-1 / TIM1 / HACVR1 protein (Catalog#90006-C07H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 242
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Cynomolgus KIM-1 / TIM1 / HACVR1 (rC KIM-1 / TIM1 / HACVR1; Catalog#90006-C07H; Asp 20-Val 135).

KIM-1 / TIM1 / HACVR1 Antibody Usage Guide

• Specificity: Cynomolgus KIM-1 / TIM1 / HACVR1
• No cross-reactivity in ELISA with

  Human TIMD3

  Mouse KIM1
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus HAVCR1. The detection limit for Cynomolgus HAVCR1 is approximately 0.039 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

KIM-1 / TIM1 / HACVR1 Antibody Background

Hepatitis A virus cellular receptor 1 (HAVCR1), also known as T cell immunoglobulin and mucin domain-containing protein 1 (TIMD1) and T cell membrane protein 1 (TIM1), is a member of the TIM family, an immunologically important group of receptors that is conserved in vertebrates. TIM1 is widely expressed with highest levels in kidney and testis. It has been shown that mouse HAVCR1 is expressed by stimulated T-cells during primary antigen stimulation and associates with immunomodulatory properties. TIM1 is a type 1 integral membrane glycoprotein, and in the ECD contains one V-type Ig-like domain extended above the cell surface by a mucin-like domain characterized by a variable number of threonine, serine, and proline (TSP) hexameric repeats. TIM1 plays a critical role in T-helper cell development and the regulation of asthma and allergic diseases. In addition to HAV, another identified ligand for TIM1 is TIMD4. Rat TIM1 ECD shares 50% and 81% amino acid sequence homology with human and mouse TIM1 ECD rexpectively.

References

1. Silberstein, E. et al., 2001, J Virol.75 (2): 717-725.
2. Silberstein, E. et al., 2003, J Virol.77 (16): 8765-8774.
3. Tami, C. et al., 2007, J Virol.81 (7): 3437-3446.
4. Garner-Spitzer, E. et al., 2009, Vaccine.27 (2): 197-204.