Human KDM1 Gene cDNA Clone / ORF Clone

KDM1 cDNA Clone Datasheet

KDM1 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_015013.3
• cDNA Size: 2559
• Gene Synonym: AOF2; KDM1; LSD1; BHC110; KDM1A
• Source: Human

KDM1 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens lysine (K)-specific demethylase 1A DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

KDM1 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KDM1 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KDM1 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTATCTG GGAAGAAGGC GGCAGCCGCG GCGGCGGCGG CTGCAGCGGC AGCAACCGGG
61       ACGGAGGCTG GCCCTGGGAC AGCAGGCGGC TCCGAGAACG GGTCTGAGGT GGCCGCGCAG
121      CCCGCGGGCC TGTCGGGCCC AGCCGAGGTC GGGCCGGGGG CGGTGGGGGA GCGCACACCC
181      CGCAAGAAAG AGCCTCCGCG GGCCTCGCCC CCCGGGGGCC TGGCGGAACC GCCGGGGTCC
241      GCAGGGCCTC AGGCCGGCCC TACTGTCGTG CCTGGGTCTG CGACCCCCAT GGAAACTGGA
301      ATAGCAGAGA CTCCGGAGGG GCGTCGGACC AGCCGGCGCA AGCGGGCGAA GGTAGAGTAC
361      AGAGAGATGG ATGAAAGCTT GGCCAACCTC TCAGAAGATG AGTATTATTC AGAAGAAGAG
421      AGAAATGCCA AAGCAGAGAA GGAAAAGAAG CTTCCCCCAC CACCCCCTCA AGCCCCACCT
481      GAGGAAGAAA ATGAAAGTGA GCCTGAAGAA CCATCGGGTG TGGAGGGCGC AGCTTTCCAG
541      AGCCGACTTC CTCATGACCG GATGACTTCT CAAGAAGCAG CCTGTTTTCC AGATATTATC
601      AGTGGACCAC AACAGACCCA GAAGGTTTTT CTTTTCATTA GAAACCGCAC ACTGCAGTTG
661      TGGTTGGATA ATCCAAAGAT TCAGCTGACA TTTGAGGCTA CTCTCCAACA ATTAGAAGCA
721      CCTTATAACA GTGATACTGT GCTTGTCCAC CGAGTTCACA GTTATTTAGA GCGTCATGGT
781      CTTATCAACT TCGGCATCTA TAAGAGGATA AAACCCCTAC CAACTAAAAA GACAGGAAAG
841      GTAATTATTA TAGGCTCTGG GGTCTCAGGC TTGGCAGCAG CTCGACAGTT ACAAAGTTTT
901      GGAATGGATG TCACACTTTT GGAAGCCAGG GATCGTGTGG GTGGACGAGT TGCCACATTT
961      CGCAAAGGAA ACTATGTAGC TGATCTTGGA GCCATGGTGG TAACAGGTCT TGGAGGGAAT
1021     CCTATGGCTG TGGTCAGCAA ACAAGTAAAT ATGGAACTGG CCAAGATCAA GCAAAAATGC
1081     CCACTTTATG AAGCCAACGG ACAAGCTGTT CCTAAAGAGA AAGATGAAAT GGTAGAGCAA
1141     GAGTTTAACC GGTTGCTAGA AGCTACATCT TACCTTAGTC ATCAACTAGA CTTCAATGTC
1201     CTCAATAATA AGCCTGTGTC CCTTGGCCAG GCATTGGAAG TTGTCATTCA GTTACAAGAG
1261     AAGCATGTCA AAGATGAGCA GATTGAACAT TGGAAGAAGA TAGTGAAAAC TCAGGAAGAA
1321     TTGAAAGAAC TTCTTAATAA GATGGTAAAT TTGAAAGAGA AAATTAAAGA ACTCCATCAG
1381     CAATACAAAG AAGCATCTGA AGTAAAGCCA CCCAGAGATA TTACTGCCGA GTTCTTAGTG
1441     AAAAGCAAAC ACAGGGATCT GACCGCCCTA TGCAAGGAAT ATGATGAATT AGCTGAAACA
1501     CAAGGAAAGC TAGAAGAAAA ACTTCAGGAG TTGGAAGCGA ATCCCCCAAG TGATGTATAT
1561     CTCTCATCAA GAGACAGACA AATACTTGAT TGGCATTTTG CAAATCTTGA ATTTGCTAAT
1621     GCCACACCTC TCTCAACTCT CTCCCTTAAG CACTGGGATC AGGATGATGA CTTTGAGTTC
1681     ACTGGCAGCC ACCTGACAGT AAGGAATGGC TACTCGTGTG TGCCTGTGGC TTTAGCAGAA
1741     GGCCTAGACA TTAAACTGAA TACAGCAGTG CGACAGGTTC GCTACACGGC TTCAGGATGT
1801     GAAGTGATAG CTGTGAATAC CCGCTCCACG AGTCAAACCT TTATTTATAA ATGCGACGCA
1861     GTTCTCTGTA CCCTTCCCCT GGGTGTGCTG AAGCAGCAGC CACCAGCCGT TCAGTTTGTG
1921     CCACCTCTCC CTGAGTGGAA AACATCTGCA GTCCAAAGGA TGGGATTTGG CAACCTTAAC
1981     AAGGTGGTGT TGTGTTTTGA TCGGGTGTTC TGGGATCCAA GTGTCAATTT GTTCGGGCAT
2041     GTTGGCAGTA CGACTGCCAG CAGGGGTGAG CTCTTCCTCT TCTGGAACCT CTATAAAGCT
2101     CCAATACTGT TGGCACTAGT GGCAGGAGAA GCTGCTGGTA TCATGGAAAA CATAAGTGAC
2161     GATGTGATTG TTGGCCGATG CCTGGCCATT CTCAAAGGGA TTTTTGGTAG CAGTGCAGTA
2221     CCTCAGCCCA AAGAAACTGT GGTGTCTCGT TGGCGTGCTG ATCCCTGGGC TCGGGGCTCT
2281     TATTCCTATG TTGCTGCAGG ATCATCTGGA AATGACTATG ATTTAATGGC TCAGCCAATC
2341     ACTCCTGGCC CCTCGATTCC AGGTGCCCCA CAGCCGATTC CACGACTCTT CTTTGCGGGA
2401     GAACATACGA TCCGTAACTA CCCAGCCACA GTGCATGGTG CTCTGCTGAG TGGGCTGCGA
2461     GAAGCGGGAA GAATTGCAGA CCAGTTTTTG GGGGCCATGT ATACGCTGCC TCGCCAGGCC
2521     ACACCAGGTG TTCCTGCACA GCAGTCCCCA AGCATGTGA

 

Quality Control:cDNA sequencing results is available upon request.