Human KCNMB3 transcript variant 4 Gene cDNA Clone / ORF Clone

KCNMB3 cDNA Clone Datasheet

KCNMB3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_014407.3
• cDNA Size: 840
• Gene Synonym: HBETA3; KCNMB2; KCNMBL; BKBETA3; SLOBETA3;KCNMB3
• Source: Human

KCNMB3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3, transcript variant 4 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 385 T/C resulting in the amino acid Phe substitution by Leu.

KCNMB3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KCNMB3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KCNMB3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGACTTTT CACCAAGCTC TGAACTGGGA TTTCATTTTG TTGCATTCAT CCTGCTCACG
61       AGACACAGGA CAGCCTTTCC TGCCTCAGGG AAGAAGAGAG AGACAGACTA CAGTGATGGA
121      GACCCACTAG ATGTGCACAA GAGGCTGCCA TCCAGTGCTG GAGAGGACCG AGCCGTGATG
181      CTGGGGTTTG CCATGATGGG CTTCTCAGTC CTAATGTTCT TCTTGCTCGG AACAACCATT
241      CTAAAGCCTT TTATGCTCAG CATTCAGAGA GAAGAATCGA CCTGCACTGC CATCCACACA
301      GATATCATGG ACGACTGGCT GGACTGTGCC TTCACCTGTG GTGTGCACTG CCACGGTCAG
361      GGGAAGTACC CGTGTCTTCA GGTGCTTGTG AACCTCAGCC ATCCAGGTCA GAAAGCTCTC
421      CTACATTATA ATGAAGAGGC TGTCCAGATA AATCCCAAGT GCTTTTACAC ACCTAAGTGC
481      CACCAAGATA GAAATGATTT GCTCAACAGT GCTCTGGACA TAAAAGAATT CTTCGATCAC
541      AAAAATGGAA CCCCCTTTTC ATGCTTCTAC AGTCCAGCCA GCCAATCTGA AGATGTCATT
601      CTTATAAAAA AGTATGACCA AATGGCTATC TTCCACTGTT TATTTTGGCC TTCACTGACT
661      CTGCTAGGTG GTGCCCTGAT TGTTGGCATG GTGAGATTAA CACAACACCT GTCCTTACTG
721      TGTGAAAAAT ATAGCACTGT AGTCAGAGAT GAGGTAGGTG GAAAAGTACC TTATATAGAA
781      CAGCATCAGT TCAAACTGTG CATTATGAGG AGGAGCAAAG GAAGAGCAGA GAAATCTTAA

 

Quality Control:cDNA sequencing results is available upon request.