Human KCNMB3 transcript variant 3 Gene cDNA Clone / ORF Clone

KCNMB3 cDNA Clone Datasheet

KCNMB3 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_171830.1
• cDNA Size: 828
• Gene Synonym: HBETA3; KCNMB2; KCNMBL; BKBETA3; SLOBETA3;KCNMB3
• Source: Human

KCNMB3 cDNA Clone Description

• cDNA Description: ORF Clone of Homo sapiens potassium large conductance calcium-activated channel, subfamily M beta member 3, transcript variant 3 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 10 C/G, resulting in the amino acid Leu substitution by Val.

KCNMB3 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

KCNMB3 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

KCNMB3 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTTCCCCG TTCTTTATGA GCTCACTGCA GTATCTCCTT CTCCCTTTCC CCAAAGGACA
61       GCCTTTCCTG CCTCAGGGAA GAAGAGAGAG ACAGACTACA GTGATGGAGA CCCACTAGAT
121      GTGCACAAGA GGCTGCCATC CAGTGCTGGA GAGGACCGAG CCGTGATGCT GGGGTTTGCC
181      ATGATGGGCT TCTCAGTCCT AATGTTCTTC TTGCTCGGAA CAACCATTCT AAAGCCTTTT
241      ATGCTCAGCA TTCAGAGAGA AGAATCGACC TGCACTGCCA TCCACACAGA TATCATGGAC
301      GACTGGCTGG ACTGTGCCTT CACCTGTGGT GTGCACTGCC ACGGTCAGGG GAAGTACCCG
361      TGTCTTCAGG TGTTTGTGAA CCTCAGCCAT CCAGGTCAGA AAGCTCTCCT ACATTATAAT
421      GAAGAGGCTG TCCAGATAAA TCCCAAGTGC TTTTACACAC CTAAGTGCCA CCAAGATAGA
481      AATGATTTGC TCAACAGTGC TCTGGACATA AAAGAATTCT TCGATCACAA AAATGGAACC
541      CCCTTTTCAT GCTTCTACAG TCCAGCCAGC CAATCTGAAG ATGTCATTCT TATAAAAAAG
601      TATGACCAAA TGGCTATCTT CCACTGTTTA TTTTGGCCTT CACTGACTCT GCTAGGTGGT
661      GCCCTGATTG TTGGCATGGT GAGATTAACA CAACACCTGT CCTTACTGTG TGAAAAATAT
721      AGCACTGTAG TCAGAGATGA GGTAGGTGGA AAAGTACCTT ATATAGAACA GCATCAGTTC
781      AAACTGTGCA TTATGAGGAG GAGCAAAGGA AGAGCAGAGA AATCTTAA

 

Quality Control:cDNA sequencing results is available upon request.