Anti-JNK2 Antibody

Cat: 10745-R011
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Anti-JNK2 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-JNK2 Antibody
Validated applications
WB,ELISA,IHC-P,ICC/IF,IF,IP
Species reactivity
Reacts with: Human
Specificity
Human JNK2
Immunogen
Recombinant Human JNK2 / MAPK9 protein (Catalog#10745-H08B)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human JNK2 / MAPK9 (rh JNK2 / MAPK9; Catalog#10745-H08B; NP_002743.3; Met 1-Arg 424).
Source
Monoclonal Rabbit IgG Clone #011
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-JNK2 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:500-1:2500  
ICC-IF 1:100-1:500  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-JNK2 Antibody (Rabbit Monoclonal antibody) Images
Human JNK2/MAPK9 Immunofluorescence(IF) 2886
Immunofluorescence staining of Human MAPK9 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human MAPK9 monoclonal antibody (1:300) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and Nucleus.
Human JNK2/MAPK9 Immunohistochemistry(IHC) 3905
Immunochemical staining of human MAPK9 in human kidney with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human JNK2/MAPK9 Immunohistochemistry(IHC) 3906
Immunochemical staining of human MAPK9 in human brain with rabbit mnoclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human JNK2/MAPK9 Western blot (WB) 13270

Anti-MAPK9 rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:48 kDa

Observed band size:54 kDa

(We are unsure as to the identity of these extra bands.)

Human JNK2/MAPK9 Immunoprecipitation(IP) 17676

MAPK9 was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg Hela Whole Cell Lysate

Lane C:0.5 mg Jurkat Whole Cell Lysate

Lane D:0.5 mg MCF-7 Whole Cell Lysate

0.5 µL anti-MAPK9 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-MAPK9 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 44 kDa

Observed band size: 44 kDa

Anti-JNK2 Antibody Alternative Names
Anti-JNK-55 Antibody;Anti-JNK2 Antibody;Anti-JNK2A Antibody;Anti-JNK2ALPHA Antibody;Anti-JNK2B Antibody;Anti-JNK2BETA Antibody;Anti-p54a Antibody;Anti-p54aSAPK Antibody;Anti-PRKM9 Antibody;Anti-SAPK Antibody;Anti-SAPK1a Antibody
JNK2 Background Information

Mitogen-activated protein kinase 9 (MAPK9), also well known as c-Jun N-terminal kinase (JNK2), is a member of MAP kinase subfamily belonging to the protein kinase superfamily. MAPK9 responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, such as c-Jun and ATF2. The crystal structure of human JNK2 complexed with an indazole inhibitor by applying a high-throughput protein engineering and surface-site mutagenesis approach. A novel conformation of the activation loop is observed, which is not compatible with its phosphorylation by upstream kinases. This activation inhibitory conformation of JNK2 is stabilized by the MAP kinase insert that interacts with the activation loop in an induced-fit manner. It suggest that the MAP kinase insert of JNK2 plays a role in the regulation of JNK2 activation, possibly by interacting with intracellular binding partners. JNK2 deficiency leads to reduced c-Jun degradation, thereby augmenting c-Jun levels and cellular proliferation, and suggests that JNK2 is a negative regulator of cellular proliferation in multiple cell types. JNK2 prevents replicative stress by coordinating cell cycle progression and DNA damage repair mechanisms. JNK2 blocks the ubiquitination of tumor suppressor p53, and thus increases the stability of p53 in nonstressed cells. JNK2 negatively regulates antigen-specific CD8+ T cell expansion and effector function, and thus selectively blocking JNK2 in CD8+ T cells may potentially enhance anti-tumor immune response. Lack of JNK2 expression was associated with higher tumor aneuploidy and reduced DNA damage response. Additionally,the JNK2 protein could be a novel therapeutic target in dry eye disease, and may provide a novel target for prevention of vascular disease and atherosclerosis.

Full Name
mitogen-activated protein kinase 9
Research Areas
References
  • Sabapathy K, et al. (2004) JNK2: a negative regulator of cellular proliferation. Cell Cycle. 3(12): 1520-3.
  • Tao J, et al. (2007) JNK2 negatively regulates CD8+ T cell effector function and anti-tumor immune response. Eur J Immunol. 37(3): 818-29.
  • Shaw D, et al. (2008) The crystal structure of JNK2 reveals conformational flexibility in the MAP kinase insert and indicates its involvement in the regulation of catalytic activity. J Mol Biol. 383(4): 885-93.
  • Osto E, et al. (2008) c-Jun N-terminal kinase 2 deficiency protects against hypercholesterolemia-induced endothelial dysfunction and oxidative stress. 118(20): 2073-80.
  • De Paiva CS, et al. (2009) Essential role for c-Jun N-terminal kinase 2 in corneal epithelial response to desiccating stress. Arch Ophthalmol. 127(12): 1625-31.
  • Chen P, et al. (2010) Jnk2 effects on tumor development, genetic instability and replicative stress in an oncogene-driven mouse mammary tumor model. PLoS One. 5(5): e10443.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-JNK2 Antibody Rabbit Polyclonal ICC/IF,IF 100961-T02
    Anti-JNK2 Antibody Rabbit Polyclonal ELISA,IP 10745-T20
    Anti-JNK2 Antibody Mouse Monoclonal FCM,ICC/IF,IF 10745-MM06
    Anti-JNK2 Antibody Rabbit Polyclonal ELISA 10745-RP01
    Anti-JNK2 Antibody Rabbit Monoclonal WB,ELISA,ICC/IF,IF,IP 10745-R004
    Anti-JNK2 Antibody Rabbit Polyclonal IHC-P,ICC/IF,IF 100961-T10
    Anti-JNK2 Antibody Mouse Monoclonal WB,ELISA,IP 10745-MM01
    Anti-JNK2 Antibody Rabbit Polyclonal ELISA,IP 10745-RP02
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