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Mouse MAPK8 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| MG50009-M |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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MAPK8 cDNA Clone Datasheet
MAPK8 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_016700.3 |
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cDNA Size: |
1155 |
| Gene Synonym: |
JNK; JNK1; Prkm8; SAPK1; AI849689 |
| Source: | Mouse |
MAPK8 cDNA Clone Description
|
cDNA Description: |
ORF Clone of Mus musculus mitogen-activated protein kinase 8 DNA. |
| Vector: | Cloning Vector: pMD18-T Simple Vector.
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for two point mutations: 207A/C and 621T/C not causing the amino acid variation. |
MAPK8 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
MAPK8 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O . Ensure the filter paper is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the scattered filter paper from the elute. The elute now contains the purified DNA.
MAPK8 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGAGCAGAA GCAAACGTGA CAACAATTTT TATAGTGTAG AGATTGGAGA TTCTACATTC 61 ACAGTCCTAA AACGATACCA GAATTTAAAG CCTATAGGCT CAGGAGCTCA AGGAATAGTG 121 TGTGCAGCTT ATGATGCCAT TCTTGAAAGA AATGTTGCAA TCAAGAAGCT CAGCCGGCCA 181 TTTCAGAATC AGACCCATGC TAAGCGCGCC TACCGAGAAC TAGTTCTTAT GAAGTGTGTT 241 AATCACAAAA ATATAATTGG CCTTTTGAAT GTTTTCACAC CACAGAAATC CCTAGAAGAA 301 TTTCAAGATG TTTACATAGT CATGGAGCTC ATGGATGCAA ATCTTTGCCA AGTGATTCAG 361 ATGGAGTTAG ATCATGAAAG AATGTCCTAC CTTCTCTATC AAATGCTGTG TGGAATCAAG 421 CACCTTCACT CTGCTGGAAT TATTCATCGG GACTTAAAGC CTAGTAATAT AGTAGTCAAA 481 TCAGACTGCA CTTTGAAGAT TCTTGATTTT GGACTGGCGA GGACTGCAGG AACGAGTTTT 541 ATGATGACGC CTTATGTGGT GACTCGCTAC TACAGAGCAC CAGAGGTCAT TCTCGGCATG 601 GGCTACAAGG AGAACGTGGA CTTATGGTCT GTGGGGTGCA TTATGGGAGA AATGGTTTGC 661 CACAAAATCC TCTTTCCAGG AAGGGACTAT ATTGATCAGT GGAATAAAGT TATTGAACAG 721 CTCGGAACAC CTTGTCCTGA ATTCATGAAG AAACTACAAC CAACAGTAAG GACTTATGTT 781 GAAAACAGGC CTAAATACGC TGGATATAGC TTTGAGAAAC TGTTCCCCGA TGTGCTTTTC 841 CCAGCTGACT CAGAGCATAA CAAACTTAAA GCCAGTCAGG CAAGAGATTT GTTATCCAAA 901 ATGCTAGTAA TAGATGCATC CAAAAGGATC TCCGTAGATG AAGCTCTCCA GCACCCATAC 961 ATCAACGTCT GGTATGATCC TTCAGAAGCA GAAGCCCCAC CACCAAAGAT CCCGGACAAG 1021 CAGTTAGATG AGAGGGAGCA CACAATAGAG GAGTGGAAAG AACTGATATA CAAGGAGGTA 1081 ATGGATTTGG AGGAACGAAC TAAGAATGGA GTCATAAGAG GGCAGCCGTC TCCTTTAGCA 1141 CAGGTGCAGC AATGA
Quality Control:cDNA sequencing results is available upon request.
