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Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone in cloning vector

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Human MAPK8 cDNA Clone Product Information
NCBI RefSeq:NM_001278547.1
RefSeq ORF Size:1284bp
cDNA Description:Full length Clone DNA of Homo sapiens mitogen-activated protein kinase 8, transcript variant JNK1-b2.
Gene Synonym:JNK, JNK1, PRKM8, SAPK1, JNK1A2, JNK21B1/2
Species:Human
Vector:pMD18-T Simple Vector
Plasmid:pMD-MAPK8JNK1beta2
Restriction Site:
Tag Sequence:
Sequence Description:Identical with the Gene Bank Ref. ID sequence.
Sequencing primers:
Promoter:
Application:
Antibiotic in E.coli:Ampicilin
Antibiotic in mammalian cell:
Shipping_carrier:Each tube contains lyophilized plasmid.
Storage:The lyophilized plasmid can be stored at room temperature for three months.
pMD18-T Simple Vector Information

pMD18-T Simple Vector is a high-efficiency TA cloning vector constructed from pUC18, of which the initial multiple cloning sites (MCS) were destroyed. Thus the cDNA should be amplified by PCR with primers containing a restriction site for subclone. Competent cells appropriate for pUC18 are also appropriated for the Vector, e.g. JM109, DH5α, TOP10. The pMD18-T Simple Vector is 2.6kb in size. Selection of the plasmid in E. coli is conferred by the ampicillin resistance gene. The coding sequence was inserted by TA cloning at site 425.

pMD18-T Simple Usage Suggestion

The coding sequence can be amplified by PCR with M13-47 and RV-M primers.

Vector Sequence Download
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone in cloning vector on other vectors
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-GFPSpark tagHG10795-ACG$225
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Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-GFPSpark tagHG10795-ANG$225
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-OFPSpark tagHG10795-ANR$225
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Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-His tagHG10795-CH$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-Myc tagHG10795-CM$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-HA tagHG10795-CY$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, C-Flag tagHG10795-M-F$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmidHG10795-M-N$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-Flag tagHG10795-NF$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-His tagHG10795-NH$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-Myc tagHG10795-NM$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmid, N-HA tagHG10795-NY$195
Human JNK1 / MAPK8 transcript variant JNK1-b2 Gene ORF cDNA clone expression plasmidHG10795-UT$195
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Background

Mitogen-activated protein kinase 8 (MAPK8), also known as JNK1, is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The protein kinases JNK1 has been found to serve as critical molecular links between obesity, metabolic inflammation, and disorders of glucose homeostasis. It is critically involved in the promotion of diet-induced obesity, metabolic inflammation and beta-cell dysfunction. The selective deficiency of JNK1 in the murine nervous system is sufficient to suppress diet-induced obesity. Genetic analysis indicates that the effects of JNK1 can be separated from effects of JNK1 on obesity. JNK1 is a potential pharmacological target for the development of drugs that might be useful for the treatment of metabolic syndrome, and type 2 diabetes. Furthermore, JNK1 plays a major role in the hypoxic cellular damage. JNK1 protein might be an attractive target for antihypoxic therapy in increasing resistance to many pathological conditions and diseases, leading to the oxygen deficit.

References
  • Betigeri S, et al. (2006) JNK1 as a molecular target to limit cellular mortality under hypoxia. Mol Pharm. 3(4): 424-30.
  • Solinas G, et al. (2010) JNK1 and IKKbeta: molecular links between obesity and metabolic dysfunction. FASEB J. 24(8): 2596-611.
  • Sabio G, et al. (2010) Role of the hypothalamic-pituitary-thyroid axis in metabolic regulation by JNK1. Genes Dev. 24(3): 256-64.
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