JAM3 / JAM-C Protein

Junctional adhesion molecule 3 Protein Datasheet

JAM3 / JAM-C Protein Product Information

• Synonym: UNQ859, PRO1868, JAM-C, JAMC
• Protein Construction: A DNA sequence encoding the human JAM3 (Q9BX67) extracellular domain (Met 1- Asn 241) was fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Human
• Expression Host: Human Cells

JAM3 / JAM-C Protein QC Testing

• Purity: > 85 % as determined by SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 32
• Molecular Mass: The recombinant human JAM3/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 451 amino acids and has a predicted molecular mass of 51 kDa. The reduced monomer migrates as an approximately 60 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from 0.2μm filtered solution of PBS, pH7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: JAM3 / JAM-C protein

JAM3 / JAM-C Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

JAM3 / JAM-C Protein Description

Junctional adhesion molecule C, also known as Junctional adhesion molecule 3, JAM3, is a single-pass type I  membrane protein which belongs to the immunoglobulin superfamily. JAM3 is widely expressed. The highest expression of JAM3 is in placenta, brain and kidney. JAM3 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. At the protein level JAM3 displays 36 and 32% identity to JAM2 and JAM1, respectively. JAM3 participates in cell-cell adhesion distinct from tight junctions. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. JAM3 is localized in the tight junctions between high endothelial cells. Unlike other proteins in the same family, this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions.

References

1. Suzuki Y. et al.,1997, Gene. 200 (1-2): 149-56.
2. Arrate MP. et al., 2001, J Biol Chem. 276 (49): 45826-32.
3. Cunningham SA. et al., 2002, J Biol Chem. 277 (31): 27589-92.
4. Muller WA. et al., 2003, Trends Immunol. 24 (6): 327-34.