Rat JAM-B / JAM2 Gene cDNA Clone / ORF Clone

JAM2 cDNA Clone Datasheet

JAM2 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001034004.1
• cDNA Size: 897
• Gene Synonym: Jam2
• Source: Rat

JAM2 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus junctional adhesion molecule 2 DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

JAM2 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

JAM2 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

JAM2 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGCGAGGA GCCCCCAAGG CCTTCTGCTG CTGCTACTGC TGCACTACTT GATGGTCGCC
61       CTGGACTATC ATAAGGCAAA TGGATTTTCT GCATCAAAAG ACCACCGTCA AGAAGTTTCA
121      GTAATAGAGT ACCAAGAGGC TATTTTAGCT TGTAAAACCC CAAAGAAGAC CACTTCCTCC
181      AGACTAGAGT GGAAGAAGCT AGGACAGGGT GTCTCCCTGG TCTACTATCA ACAGGCTCTC
241      CAAGGCGACT TTAAAGACCG TGCGGAGATG ATAGATTTCA ATATACGAAT CAAGAATGTC
301      ACAAGACATG ATGCTGGAGA GTATCGCTGC GAAGTCAGCG CCCCGACTGA GCAAGGCCAG
361      AACCTGCAGG AAGATACAGT CATGCTGGAA GTCCTGGTGG CCCCCGCTGT CCCTTCATGT
421      GAAGTACCTA CTTCCGTTAT GAGTGGGAGT GTTGTGGAGC TACGGTGTCA AGAAAAAGAA
481      GGAAATCCAG CCCCTGAGTA CATATGGTTT AAAGATGGCA CAAGTTTGCT GGGCAATCCA
541      AAGGGCGGCG CACACAGGAA CAGCTCGTAC ACAATGAATA CAAAATCTGG AACTCTGCAA
601      TTCAACATGA TCTCCAAGAT GGACAGTGGA GAGTATTACT GTGAAGCCCG CAACTCTGTT
661      GGGCATCGCA GGTGTCCCGG GAAACGGATG CAAGTAGATG TTCTCAACAT AAGTGGCATC
721      ATAGCAGCTG TGGTTGTTGT GGCCTTCGTC ATTTCTGCGT GTGGCCTTAG TATATGCTAT
781      GCTCAGAGAA AGGGCTACTT TTCAAAAGAA ACTTCCTTCC AGAAGGGCAG TCCTGCATCT
841      AAAGCCACTA CAATGAGCGA AAATGATTTC AAGCACACAA AATCCTTTAT AATTTAA

 

Quality Control:cDNA sequencing results is available upon request.